Team:Goettingen/week8-1

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<h2><b>VX_06_18 </b></h2><br>
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<h2><b>V06_18 </b></h2><br>
<b>V-Gradiant agar plates (Variation of V06_14)</b><br>
<b>V-Gradiant agar plates (Variation of V06_14)</b><br>
<ul>
<ul>
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<li>Experiment: <br>This time, the plates were poured in three steps. This should result in a more directed, triangular gradient. Furthermore, the bacteria were dropped on radom spots, to identify the best dropping position.</li>
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<li>Experiment: <br>This time, the plates were poured in three steps. This should result in a more directed, triangular gradient. Furthermore, the bacteria were dropped on random spots, to identify the best dropping position.</li>
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<b>Swimmimg assay with M9-agar and Whatman-paper</b><br>
<b>Swimmimg assay with M9-agar and Whatman-paper</b><br>
<ul>
<ul>
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<li>Experiment: <br> "M9 agar plates" (4 mL 100% glycerol, 80 mL 5x M9 salt solution, 400 µL CaCl2*2H20 [20 mg/mL], 400 µL MgSO4 [0.12 g/mL], 0.3% agar, add 400 mL H2O) were produced and used for swimming assays, as explained in methods. The whatman papers was incorporated with 100 µL of a 50 mM aspartate solution.</li>
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<li>Experiment: <br> "M9 agar plates" (4 mL 100% glycerol, 80 mL 5x M9 salt solution, 400 µL CaCl2*2H20 [20 mg/mL], 400 µL MgSO4 [0.12 g/mL], 0.3% agar, add 400 mL H2O) were produced and used for swimming assays, as explained in methods. The Whatman papers was incorporated with 100 µL of a 50 mM aspartate solution.</li>
<li> Observations & Results:<br>
<li> Observations & Results:<br>
After about 15h were no swimming visible. The only exception was the BL21 strain, which showed a little swimming.  
After about 15h were no swimming visible. The only exception was the BL21 strain, which showed a little swimming.  
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<h2><b>V06_26 </b></h2><br>
 
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<b>Separation assay</b><br>
 
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<li>Experiment: <br>Similar to V06_13, strains with different promotor were mixed 1:1 and dropped onto the plates</li>
 
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Latest revision as of 18:29, 26 September 2012

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#1 Selection / Swimming - 8th Week

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V06_18


V-Gradiant agar plates (Variation of V06_14)
  • Experiment:
    This time, the plates were poured in three steps. This should result in a more directed, triangular gradient. Furthermore, the bacteria were dropped on random spots, to identify the best dropping position.

V06_21


Swimmimg assay with M9-agar and Whatman-paper
  • Experiment:
    "M9 agar plates" (4 mL 100% glycerol, 80 mL 5x M9 salt solution, 400 µL CaCl2*2H20 [20 mg/mL], 400 µL MgSO4 [0.12 g/mL], 0.3% agar, add 400 mL H2O) were produced and used for swimming assays, as explained in methods. The Whatman papers was incorporated with 100 µL of a 50 mM aspartate solution.
  • Observations & Results:
    After about 15h were no swimming visible. The only exception was the BL21 strain, which showed a little swimming.


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