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Team:NTU-Taida/Parts
From 2012.igem.org
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==Sequence== | ==Sequence== | ||
This is test.This is test.This is test.This is test.This is test. | This is test.This is test.This is test.This is test.This is test. | ||
| + | |||
| + | p_-:plasmid-free ratio | ||
| + | p_+:plasmid retained ratio | ||
| + | D:dilution rate | ||
| + | R: plasmid loss rate(R= Mutation rate + Segregational instability) | ||
| + | μ:growth rate | ||
| + | |||
{| class="table table-hover table-bordered" | {| class="table table-hover table-bordered" | ||
Revision as of 18:18, 26 September 2012
Contents |
CPP
Sequence
This is test.This is test.This is test.This is test.This is test.
p_-:plasmid-free ratio p_+:plasmid retained ratio D:dilution rate R: plasmid loss rate(R= Mutation rate + Segregational instability) μ:growth rate
| 标题文字 | 标题文字 | 标题文字 |
|---|---|---|
| 示例 | 示例 | 示例 |
| 示例 | 示例 | 示例 |
| 示例 | 示例 | 示例 |
File:NTU-Taida-Suicide-37-25c.gif
|
Parameter |
Description |
Value |
Reference |
|
αTetR |
TetR max. production rate |
0.8 μM/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref5" title="Team:ETH Zurich/Modeling/References">[5]</a> |
|
αFadR |
FadR max. production rate |
100 μM/min |
a. |
|
αGLP-1 |
GLP-1 max. production rate |
1.23 μM/min |
a. |
|
αLacI |
LacI max. production rate |
0.8 μM/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
ks1 |
AHLi production rate |
0.01 1/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a> |
|
ρR |
LuxR-AHL dimerization rate |
0.5 1/(μM3*min) |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
βTetR |
TetR repression coefficient |
0.13 μM |
a. |
|
βFA |
FA repression coefficient |
10 μM |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
βFadR |
FadR repression coefficient |
0.13 μM |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
βLacI |
LacI repression coefficient |
0.8 μM |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
βR |
LuxR-AHL repression coefficient |
0.01 μM |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
γTetR |
TetR degradation rate |
0.0692 1/min |
a. |
|
γLacI |
LacI degradation rate |
0.0231 1/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
γGLP-1 |
GLP-1 degradation rate |
0.0731/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
γLuxI |
LuxI degradation rate |
0.0167 1/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref3" title="Team:ETH Zurich/Modeling/References">[3]</a> |
|
ks0 |
AHLi degradation rate |
1 1/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a> |
|
kse |
AHLe degradation rate |
1 1/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a> |
|
γR |
LuxR-AHL degradation rate |
0.0231 1/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
γRFP |
RFP degradation rate |
0.0041 1/min |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref4" title="Team:ETH Zurich/Modeling/References">[4]</a> |
|
n1 |
FadR cooperativity coefficient |
3 |
a. |
|
n2 |
FA cooperativity coefficient |
2 |
a. |
|
n3 |
LuxR-AHL cooperativity coefficient |
3 |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
n5 |
TetR cooperativity coefficient |
2 |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a> |
|
n6 |
LacI cooperativity coefficient |
4 |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a> |
|
η |
AHL Diffusion rate across the cell membrane |
2 |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a> |
|
ηext |
Average diffusion rate for all cells |
1 |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a> |
|
Parameter<o:p></o:p> |
Description <o:p></o:p> |
Value <o:p></o:p> |
Reference<o:p></o:p> |
|
αTetR<o:p></o:p> |
TetR max. production rate<o:p></o:p> |
0.8 μM/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref5" title="Team:ETH Zurich/Modeling/References">[5]</a><o:p></o:p> |
|
αFadR<o:p></o:p> |
FadR max. production rate<o:p></o:p> |
100 μM/min<o:p></o:p> |
a.<o:p></o:p> |
|
αGLP-1<o:p></o:p> |
GLP-1 max. production rate<o:p></o:p> |
1.23 μM/min<o:p></o:p> |
a.<o:p></o:p> |
|
αLacI<o:p></o:p> |
LacI max. production rate<o:p></o:p> |
0.8 μM/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
ks1<o:p></o:p> |
AHLi production rate<o:p></o:p> |
0.01 1/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a><o:p></o:p> |
|
ρR<o:p></o:p> |
LuxR-AHL dimerization rate<o:p></o:p> |
0.5 1/(μM3*min)<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
βTetR<o:p></o:p> |
TetR repression coefficient<o:p></o:p> |
0.13 μM<o:p></o:p> |
a.<o:p></o:p> |
|
βFA<o:p></o:p> |
FA repression coefficient<o:p></o:p> |
10 μM<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
βFadR<o:p></o:p> |
FadR repression coefficient<o:p></o:p> |
0.13 μM<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
βLacI<o:p></o:p> |
LacI repression coefficient<o:p></o:p> |
0.8 μM<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
βR<o:p></o:p> |
LuxR-AHL repression coefficient<o:p></o:p> |
0.01 μM<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
γTetR<o:p></o:p> |
TetR degradation rate<o:p></o:p> |
0.0692 1/min<o:p></o:p> |
a.<o:p></o:p> |
|
γLacI<o:p></o:p> |
LacI degradation rate<o:p></o:p> |
0.0231 1/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
γGLP-1<o:p></o:p> |
GLP-1 degradation rate<o:p></o:p> |
0.0731/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
γLuxI<o:p></o:p> |
LuxI degradation rate<o:p></o:p> |
0.0167 1/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref3" title="Team:ETH Zurich/Modeling/References">[3]</a><o:p></o:p> |
|
ks0<o:p></o:p> |
AHLi degradation rate<o:p></o:p> |
1 1/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a><o:p></o:p> |
|
kse<o:p></o:p> |
AHLe degradation rate<o:p></o:p> |
1 1/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a><o:p></o:p> |
|
γR<o:p></o:p> |
LuxR-AHL degradation rate<o:p></o:p> |
0.0231 1/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
γRFP<o:p></o:p> |
RFP degradation rate<o:p></o:p> |
0.0041 1/min<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref4" title="Team:ETH Zurich/Modeling/References">[4]</a><o:p></o:p> |
|
n1<o:p></o:p> |
FadR cooperativity coefficient<o:p></o:p> |
3<o:p></o:p> |
a.<o:p></o:p> |
|
n2<o:p></o:p> |
FA cooperativity coefficient<o:p></o:p> |
2<o:p></o:p> |
a.<o:p></o:p> |
|
n3<o:p></o:p> |
LuxR-AHL cooperativity coefficient<o:p></o:p> |
3<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
n5<o:p></o:p> |
TetR cooperativity coefficient<o:p></o:p> |
2<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a><o:p></o:p> |
|
n6<o:p></o:p> |
LacI cooperativity coefficient<o:p></o:p> |
4<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref1" title="Team:ETH Zurich/Modeling/References">[1]</a><o:p></o:p> |
|
η<o:p></o:p> |
AHL Diffusion rate across the cell membrane<o:p></o:p> |
2<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a><o:p></o:p> |
|
ηext<o:p></o:p> |
Average diffusion rate for all cells<o:p></o:p> |
1<o:p></o:p> |
<a href="https://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref2" title="Team:ETH Zurich/Modeling/References">[2]</a><o:p></o:p> |
GLP
Sequence
This is test.This is test.This is test.
pseudomonas putida mt-2 chromosome parAB cassette
Sequence
Phs thermal promoter
Sequence
This is test. This is test.This is test.This is test.This is test.This is test.This is test.This is test.This is test.This is test.This is test.This is test.This is test.This is test.This is test.
pseudomonas putida mt-2 chromosome partition parB binding site
Sequence
gamma delta resolution system cassette
Sequence
F plasmid srnBC toxin-antitoxin cassette
Sequence
