Team:Paris Bettencourt/Overview
From 2012.igem.org
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- | <a class="thumbnail" href="#thumb"><img src="/wiki/images/5/5c/RestrictionSystem.png" width="60px" border="0" /><span><img src="/wiki/images/9/97/Restriction2PB12.gif" width="550px" /><br /><div id="txtOV"> | + | <a class="thumbnail" href="#thumb"><img src="/wiki/images/5/5c/RestrictionSystem.png" width="60px" border="0" /><span><img src="/wiki/images/9/97/Restriction2PB12.gif" width="550px" /><br /><div id="txtOV">Restriction Enzyme system system.</div></span></a> |
- | <a class="thumbnail" href="#thumb"><img src="https://static.igem.org/mediawiki/2012/7/78/ToxinAntitoxin.png" width="60px" border="0" /><span><img src="/wiki/images/8/8f/Toxin3aPB12.gif" width="550px"/><br /><div id="txtOV"> | + | <a class="thumbnail" href="#thumb"><img src="https://static.igem.org/mediawiki/2012/7/78/ToxinAntitoxin.png" width="60px" border="0" /><span><img src="/wiki/images/8/8f/Toxin3aPB12.gif" width="550px"/><br /><div id="txtOV">Toxin/anti-Toxin system.</div></span></a> |
<a class="thumbnail" href="#thumb"><img src="https://static.igem.org/mediawiki/2012/1/1c/SemanticContainment.png" width="60px" border="0" /><span><img src="/wiki/images/8/8f/Toxin3aPB12.gif" width="550px" /><br /><div id="txtOV">Toxin/anti-Toxin system.</div></span></a> | <a class="thumbnail" href="#thumb"><img src="https://static.igem.org/mediawiki/2012/1/1c/SemanticContainment.png" width="60px" border="0" /><span><img src="/wiki/images/8/8f/Toxin3aPB12.gif" width="550px" /><br /><div id="txtOV">Toxin/anti-Toxin system.</div></span></a> | ||
<a class="thumbnail" href="#thumb"><img src="https://static.igem.org/mediawiki/2012/1/1c/SemanticContainment.png" width="60px" border="0" /><span><img src="/wiki/images/8/8f/Toxin3aPB12.gif" width="550px" /><br /><div id="txtOV">Toxin/anti-Toxin system.</div></span></a> | <a class="thumbnail" href="#thumb"><img src="https://static.igem.org/mediawiki/2012/1/1c/SemanticContainment.png" width="60px" border="0" /><span><img src="/wiki/images/8/8f/Toxin3aPB12.gif" width="550px" /><br /><div id="txtOV">Toxin/anti-Toxin system.</div></span></a> |
Revision as of 16:45, 26 September 2012
![](/wiki/images/5/5c/RestrictionSystem.png)
![](/wiki/images/9/97/Restriction2PB12.gif)
Restriction Enzyme system system.
![](https://static.igem.org/mediawiki/2012/7/78/ToxinAntitoxin.png)
![](/wiki/images/8/8f/Toxin3aPB12.gif)
Toxin/anti-Toxin system.
![](https://static.igem.org/mediawiki/2012/1/1c/SemanticContainment.png)
![](/wiki/images/8/8f/Toxin3aPB12.gif)
Toxin/anti-Toxin system.
![](https://static.igem.org/mediawiki/2012/1/1c/SemanticContainment.png)
![](/wiki/images/8/8f/Toxin3aPB12.gif)
Toxin/anti-Toxin system.
![](https://static.igem.org/mediawiki/2012/1/1c/SemanticContainment.png)
![](/wiki/images/8/8f/Toxin3aPB12.gif)
Toxin/anti-Toxin system.
Project Overview
Contents |
Objectives
Our project aims to:
- Raise the issue of biosafety, and advocate the discerning use of biosafety circuits in future iGEM projects as a requirement
- Evaluate the risk of HGT in different SynBio applications
- Develop a new, improved containment system to expand the range of environments where GEOs can be used safely.
To do so, we:
- Engaged the general public and scientific community through debate
- Raised the question about how we can regulate this practices
- Compiled a parts page of safety circuits in the registry
- Relied on three levels of containment :
- Physical containment with alginate capsules
- Semantic containment using an amber suppressor system
- An improved killswitch featuring delayed population-level suicide through complete genome degradation.
We strived to make our system as robust against mutations as possible.
General recommandation for a good killswitch device
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Step 1
Step 2
Step 2.
Step 3
Step 3.