Team:TMU-Tokyo/Notebook/Experiment
From 2012.igem.org
Line 57: | Line 57: | ||
<Hr><Br> | <Hr><Br> | ||
</p> | </p> | ||
- | + | <p class="description">Experiment Overview</p> | |
<p class="description3"> | <p class="description3"> | ||
<b>■Week 0(8.9-8.11)</b><Br> | <b>■Week 0(8.9-8.11)</b><Br> | ||
Line 95: | Line 95: | ||
<p class="description"> | <p class="description"> | ||
<b>■7th week (9.24 - 9.30)</b><Br> | <b>■7th week (9.24 - 9.30)</b><Br> | ||
- | Assay→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Experiment/7th_week_(9.24_-_9.30)">more</A> and <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay">Assay</A><Br></p> | + | Assay→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Experiment/7th_week_(9.24_-_9.30)">more</A> and <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay">Assay protocol</A><Br></p> |
<Br> | <Br> | ||
<Br> | <Br> |
Revision as of 14:21, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
Plasmid DNA Purification
Genome DNA Purification
Restruction Enzyme Degestion
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Experiment
Experiment Overview
■Week 0(8.9-8.11)
■ 9th Aug
Preparation of culture plates with Ampicillin
■ 10th Aug
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate
■ 11th Aug
Confirmation of the E.coli growth
Cultivation of the E.coli on LB culture
■1st week (8.13 - 8.19)
Make Device3→more
■2nd week (8.20 - 8.26)
Make Device1→more
■3rd week (8.27 - 9. 2)
Make Device1 and Make Device3→more
■4th week (9. 3 - 9. 9)
Make all Device→more
■5th week (9.10 - 9.16)
Make all Device→more
■6th week (9.17 - 9.23)
Make all Device→more
■7th week (9.24 - 9.30)
Assay→more and Assay protocol