Team:Goettingen/week14-3

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Revision as of 13:40, 26 September 2012


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V07_30_1 2^nd round: Analysis of the transformation V07_27

Observations & Results:
Neither the liquid culture nor the plates exhibited bacterial growth.

V07_30_2 2^nd round: Saturated mutagenesis PCR, 1000 µL

Experiment:
The PCR was set up again, seeing as we lost our DNA material during the ethanol precipitation the previous week.

V07_31_1 2^nd round: PCR clean-up

Experiment:
The PCR clean-up was performed according to the established protocol form week 10.

Observations & Results:
The correspodning agarose gel showed a band of the expected size.

V07_31_2 2^nd round: DpnI/BsaI digestion and purification

Experiment:
The digestion was performed with a total volume of 300 µL according to protocol of week 10. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!

V07_31_3 1^st round: Ligation

Experiment:
The ligation was set up in a 100 µL batch according to protocol. Incubation over night at 16 °C.

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 <b>V07_31_2 2<sup>nd</sup> round: <i>Dpn</i>I/<i>Bsa</i>I digestion and purification</b><br>
 <ul>
-<li>Experiment: <br>The digestion was performed with a total volume of 500 µL according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">protocol of week 10</a>. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!</li>
+<li>Experiment: <br>The digestion was performed with a total volume of 300 µL according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">protocol of week 10</a>. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!</li>
 </ul>
 <br>
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+b>V07_31_2 2
 b>V07_31_2 2