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| | {{Team:Bielefeld/Head}} | | {{Team:Bielefeld/Head}} |
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| - | =Labjournal=
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1 Week1]
| + | <html> |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2 Week2]
| + | <style type="text/css"> |
| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3 Week3]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4 Week4]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5 Week5]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6 Week6]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7 Week7]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8 Week8]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9 Week9]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10 Week10]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11 Week11]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12 Week12]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13 Week13]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16 Week16]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17 Week17]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18 Week18]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19 Week19]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20 Week20]
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| - | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21 Week21]
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| | + | ul {list-style-image:none;} |
| | + | #bodyContent{ |
| | + | background-color: white; |
| | + | } |
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| - | ==Week 4 (05/21 - 05/27/12)==
| + | </style> |
| | | | |
| - | ''' weekly seminar:'''
| + | <!-- navigator --> |
| - | * Lab service: Isabel
| + | <div id="nav" class="tabs"> |
| - | * We try to establish a collaboration with the iGEM team from [https://2012.igem.org/Team:SDU-Denmark SDU-Denmark]
| + | <div class="scroller"> |
| - | * Got our distribution kits
| + | <ul style="list-style-type:none"> |
| - | * First successful cloning and cultivations
| + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/Starting"><strong>Prologue</strong></a></li> |
| - | * Who wants to be a summer school teacher?
| + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1"><strong>Week 1</strong></a></li> |
| - | * We will not travel to the ACHEMA because only local teams are invited
| + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2"><strong>Week 2</strong></a></li> |
| - | * Do we want to participate in the Biolympics? (It's a sports party with fun organized by the [http://bts-ev.de/ bts])
| + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3"><strong>Week 3</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4"><strong>Week 4</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5"><strong>Week 5</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6"><strong>Week 6</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7"><strong>Week 7</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8"><strong>Week 8</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9"><strong>Week 9</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10"><strong>Week 10</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11"><strong>Week 11</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12"><strong>Week 12</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13"><strong>Week 13</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14"><strong>Week 14</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15"><strong>Week 15</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16"><strong>Week 16</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17"><strong>Week 17</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18"><strong>Week 18</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19"><strong>Week 19</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20"><strong>Week 20</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"><strong>Week 21</strong></a></li> |
| | + | <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week22"><strong>Week 22</strong></a></li> |
| | | | |
| | + | </ul> |
| | | | |
| - | ===Monday May 21st===
| + | </div> |
| - | * '''Team Modeling''': Our aims for modeling:
| + | </div> |
| | + | </html> |
| | | | |
| - | ** model the expression of the laccases in the organisms.
| + | <div style="text-align:justify;"> |
| - | ** model the aktivity of our enzyms.
| + | ==Week 4 (05/21 - 05/27/12)== |
| - | ** model the interesing parts of a clarification plant (the part witch are interesing for our cleaner.
| + | |
| - | | + | |
| - | * '''Team Bacterial Laccases''': We did some restriction digests to clone our laccase PCR products from ''Xanthomonas campestris'' and ''E. coli'' in pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in our competent KRX cells, picked colonies and made plasmidisolations. After that we ligated the digested products with the digested plasmid backbone.
| + | |
| - | | + | |
| - | ===Tuesday May 22nd=== | + | |
| - | '''Team Bacterial Laccases''': Transformation of the ligated plasmids in competent ''E. coli'' KRX cells.
| + | |
| - | | + | |
| - | ===Wednesday May 23rd=== | + | |
| - | *''' Team Student Academy:''' Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. For setup see [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th here]. Got intense red fluorescing colonies but no green fluorescing colonies. Made a backup of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]. Asking for other plasmids containing GFP at the working groups of our University.
| + | |
| - | | + | |
| - | *'''Team Bacterial Laccases''':
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| - | ** After there were no colonies on our pSB1C3 + CopA (''Xanthomonas campestris'') plate we did the transformation with the same ligation preparation again. For the other ligation with pSB1C3 + Cueo (''E. coli'') we started colony PCRSs to find postive colonies. Sadly the colony PCR showed no product.
| + | |
| - | ** Purification of the ''T. thermo'' laccase PCR product out of agarose gel.
| + | |
| | | | |
| - | ===Thursday May 24th===
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| - | *'''Team Student Academy:''' Made a liquid culture of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] at 30°C. There was no fluorescence. Furthermore [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] was transformed. Also got intense red fluorescing colonies.
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| | | | |
| - | ===Friday May 25th===
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| - | '''Team Bacterial Laccases''':
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| - | * We had to to the PCR on ''T. thermo'' laccase again, because before the amount of purified PCR product was very low.
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| | | | |
| - | ===Saturday May 26th===
| + | {{Team:Bielefeld/Sponsoren}} |
| - | ===Sunday May 27th===
| + | |
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