Team:Goettingen/week21-3

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qrtPCR with promoter constructs was performed, for a detailed protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Experimental_design">click here</a>.<br>
qrtPCR with promoter constructs was performed, for a detailed protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Experimental_design">click here</a>.<br>
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<li>Observations and results<br>
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<li>Observations and results:<br>
CP-values of triplets showed values in a range which were useful for <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Analysis_via_the_2.E2.80.93.CE.94.CE.94CT_.28Livak.29_Method">analysis via the 2–ΔΔCT (Livak) method</a> of expression rates. </li>
CP-values of triplets showed values in a range which were useful for <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Analysis_via_the_2.E2.80.93.CE.94.CE.94CT_.28Livak.29_Method">analysis via the 2–ΔΔCT (Livak) method</a> of expression rates. </li>
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Revision as of 13:02, 25 September 2012

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#3 Chemoreceptor Library - 21th Week

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V09_17


Synthesis of cDNA and qrtPCR
  • Experiment:
    cDNA synthesis of RNA from V09_16 was performed using Reverse Transcription Kit (Qiagen).
    qrtPCR with promoter constructs was performed, for a detailed protocol click here.
  • Observations and results:
    CP-values of triplets showed values in a range which were useful for analysis via the 2–ΔΔCT (Livak) method of expression rates.


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