Circuit

The main circuit is designed to detect the presence of fatty acid in the intestinal environment and produce the peptide drug (GLP-1 in this case) and cell penetrating peptide (CPP) as response. There are two core systems: double repressors and quorum sensing.

Stability and Safety

Every GM system that will function outside lab will face two major problems:system stability and safety! Without selective pressure ,we have to deal with plasmid instability ; to make our coli colonized bowel we have to use recA+ strains which may cause plasmid multimer. Our GM coli will also contact with many kinds of bacteria at the risk of horizontal gene transfer.

The following segment is our struggle against these obstacles.