<li>Observations and results: <br>The corresponding agarose gel showed bands of the expected sizes. However, we apparently did lose DNA material. Later analysis suggested that we used the wrong Kit peqGOLD Gel extraction Kit (PeqLab)) for the clean-up. </li>
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<li>Observations and results: <br>The corresponding agarose gel showed bands of the expected sizes. However, we apparently did lose DNA material. Later analysis suggested that we used the wrong Kit (peqGOLD Gel extraction Kit (PeqLab)) for this clean-up. </li>
2nd round: Analysis of plates and liquid culture V07_20
Experiment: 14 clones were transferred to a 5 mL liquid culture (LB + CM) and incubated over night at 37 °C, 180 rpm. The liquid culture was centrifuged at 4800 rpm, 4 °C, 10 min. The pellet was stored at -20 °C.
Observations and results: Both the liquid culture and the plates exhibited bacterial growth.
V07_25
V07_25_1 2nd round: Plasmid preparation clones 1-14 for subsequent sequencing
Experiment: Plasmid preparation was performd with peqGOLD Plasmid Miniprep Kit (PeqLab) following the user manual. DNA concentrations were determined via nanodrop for subsequent sequencing.
Experiment: The PCR was set up following the protocol from week 10.
Observations and results: The corresponding agarose gel showed bands of the expected sizes. However, we apparently did lose DNA material. Later analysis suggested that we used the wrong Kit (peqGOLD Gel extraction Kit (PeqLab)) for this clean-up.
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