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Team:NTU-Taida/Result
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| - | {{:Team:NTU-Taida/Templates/BSHero|Title= | + | {{:Team:NTU-Taida/Templates/BSHero|Title=Result|Content=<p>Testing Hero</p>}} |
| + | == '''Results''' == | ||
| - | == | + | ===Secretion system=== |
| - | + | ||
| - | + | ||
In order to evaluate the secretion system, we combined our products and signal peptides (SP1,SP2 and SP3). We de novo synthesized the following peptide genes: signal peptide 1(SP1)-CPP-Flag; signal peptide 2(SP2)-CPP-Flag; signal peptide 3(SP3)-CPP-Flag; SP1-GLP-1. By linking these peptide genes with constitutively-expressed promoter and ribosome-binding site, we use either Escherichia coli K12 DH5α or Escherichia coli BL-21 strain to express them. Later, we used several methods to identify the target peptides in the extracellular fluid, the outer environment. Those methods include the dot-immunoblotting, ELISA and Western blotting. Those methods’ detail description are as below and the results are being discussed in detail. | In order to evaluate the secretion system, we combined our products and signal peptides (SP1,SP2 and SP3). We de novo synthesized the following peptide genes: signal peptide 1(SP1)-CPP-Flag; signal peptide 2(SP2)-CPP-Flag; signal peptide 3(SP3)-CPP-Flag; SP1-GLP-1. By linking these peptide genes with constitutively-expressed promoter and ribosome-binding site, we use either Escherichia coli K12 DH5α or Escherichia coli BL-21 strain to express them. Later, we used several methods to identify the target peptides in the extracellular fluid, the outer environment. Those methods include the dot-immunoblotting, ELISA and Western blotting. Those methods’ detail description are as below and the results are being discussed in detail. | ||
| + | ===Dot immunoblotting=== | ||
| + | |||
| + | ===Western blotting=== | ||
| + | ===ELISA=== | ||
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Latest revision as of 13:45, 24 September 2012
Result
Testing Hero
Contents |
Results
Secretion system
In order to evaluate the secretion system, we combined our products and signal peptides (SP1,SP2 and SP3). We de novo synthesized the following peptide genes: signal peptide 1(SP1)-CPP-Flag; signal peptide 2(SP2)-CPP-Flag; signal peptide 3(SP3)-CPP-Flag; SP1-GLP-1. By linking these peptide genes with constitutively-expressed promoter and ribosome-binding site, we use either Escherichia coli K12 DH5α or Escherichia coli BL-21 strain to express them. Later, we used several methods to identify the target peptides in the extracellular fluid, the outer environment. Those methods include the dot-immunoblotting, ELISA and Western blotting. Those methods’ detail description are as below and the results are being discussed in detail.
Dot immunoblotting
Western blotting
ELISA
