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Team:Paris Bettencourt/Delay
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==Overview== | ==Overview== | ||
This part of the design will trigger the DNA degradation mechanism. To do so, we will use two different approaches. The first one, that we called "simple delay system", will be a way to trigger a restriction enzyme, which will cut the plasmid carrying the antitoxin, and thus let the cell sensitive to the toxin produced on the chromosome. In the "sRNA delay system, we use a different strategy. Here, the toxin would be cloned without the Immunity protein, in order to avoid any possible resistance to the toxin due to a non-degraded plasmid. To ensure that the toxin will not be produced while the cells are performing their function, we will use a stationary phase promoter and sRNA repression induced by arabinose. | This part of the design will trigger the DNA degradation mechanism. To do so, we will use two different approaches. The first one, that we called "simple delay system", will be a way to trigger a restriction enzyme, which will cut the plasmid carrying the antitoxin, and thus let the cell sensitive to the toxin produced on the chromosome. In the "sRNA delay system, we use a different strategy. Here, the toxin would be cloned without the Immunity protein, in order to avoid any possible resistance to the toxin due to a non-degraded plasmid. To ensure that the toxin will not be produced while the cells are performing their function, we will use a stationary phase promoter and sRNA repression induced by arabinose. | ||
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Write here the objectives of your project | Write here the objectives of your project | ||
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==Design== | ==Design== | ||
Revision as of 09:42, 24 September 2012
- Main
- Team
- Project
- Achievements
- Human Practice
- Safety
- Notebook
- Attributions
Overview
Delay system
Semantic containment
Restriction enzyme system
MAGE
Suicide system
Encapsulation
Synthetic import domain
Safety Questions
Safety AssessmentContents |
Overview
This part of the design will trigger the DNA degradation mechanism. To do so, we will use two different approaches. The first one, that we called "simple delay system", will be a way to trigger a restriction enzyme, which will cut the plasmid carrying the antitoxin, and thus let the cell sensitive to the toxin produced on the chromosome. In the "sRNA delay system, we use a different strategy. Here, the toxin would be cloned without the Immunity protein, in order to avoid any possible resistance to the toxin due to a non-degraded plasmid. To ensure that the toxin will not be produced while the cells are performing their function, we will use a stationary phase promoter and sRNA repression induced by arabinose.
Simple delay system
Write here the objectives of your project
Design
Present the design of your system, both in a written form, and a schematic one.
Experiments and results
Characterisation of X
Experimental setup
Describe the experiment
Results
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Testing of the system
Experimental setup
Describe the experiment
Results
Present your results
