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Team:NTU-Taida/Result
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=='''Results'''== | =='''Results'''== | ||
| - | === | + | ===Secretion system=== |
In order to evaluate the secretion system, we combined our products and signal peptides (SP1,SP2 and SP3). We de novo synthesized the following peptide genes: signal peptide 1(SP1)-CPP-Flag; signal peptide 2(SP2)-CPP-Flag; signal peptide 3(SP3)-CPP-Flag; SP1-GLP-1. By linking these peptide genes with constitutively-expressed promoter and ribosome-binding site, we use either Escherichia coli K12 DH5α or Escherichia coli BL-21 strain to express them. Later, we used several methods to identify the target peptides in the extracellular fluid, the outer environment. Those methods include the dot-immunoblotting, ELISA and Western blotting. Those methods’ detail description are as below and the results are being discussed in detail. | In order to evaluate the secretion system, we combined our products and signal peptides (SP1,SP2 and SP3). We de novo synthesized the following peptide genes: signal peptide 1(SP1)-CPP-Flag; signal peptide 2(SP2)-CPP-Flag; signal peptide 3(SP3)-CPP-Flag; SP1-GLP-1. By linking these peptide genes with constitutively-expressed promoter and ribosome-binding site, we use either Escherichia coli K12 DH5α or Escherichia coli BL-21 strain to express them. Later, we used several methods to identify the target peptides in the extracellular fluid, the outer environment. Those methods include the dot-immunoblotting, ELISA and Western blotting. Those methods’ detail description are as below and the results are being discussed in detail. | ||
| - | === | + | ===Dot immunoblotting=== |
| - | === | + | ===Western blotting=== |
| - | === | + | ===ELISA=== |
{{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Project}} | {{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Project}} | ||
Revision as of 07:35, 23 September 2012
Result
Testing Hero
Contents |
Results
Secretion system
In order to evaluate the secretion system, we combined our products and signal peptides (SP1,SP2 and SP3). We de novo synthesized the following peptide genes: signal peptide 1(SP1)-CPP-Flag; signal peptide 2(SP2)-CPP-Flag; signal peptide 3(SP3)-CPP-Flag; SP1-GLP-1. By linking these peptide genes with constitutively-expressed promoter and ribosome-binding site, we use either Escherichia coli K12 DH5α or Escherichia coli BL-21 strain to express them. Later, we used several methods to identify the target peptides in the extracellular fluid, the outer environment. Those methods include the dot-immunoblotting, ELISA and Western blotting. Those methods’ detail description are as below and the results are being discussed in detail.
Dot immunoblotting
Western blotting
ELISA
