Team:Goettingen/week3-2

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                              <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">&#8901; Newspaper</div></span></span></a></li>
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                              </li><li><a href="https://2012.igem.org/Team:Goettingen/Synthetic_Biology_Day"><span><span><div style="text-indent:20px;">&#8901; Synthetic Biology Day</div></span></span></a></li> -->
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                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli"><span><span>Flash Coli</span></span></a></li>
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                        <a href="https://2012.igem.org/Team:Goettingen/Sponsoring" style="color: white;">Sponsoring</a>
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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
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<table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Contents</h2> <span class="toctoggle">[<a href="javascript:toggleToc()" class="internal" id="togglelink">hide</a>]</span></div>
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<li class="toclevel-1"><a href="#week1"><span class="tocnumber">1</span> <span class="toctext">3rd week (14th to 20th May)</span></a></li>
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<li class="toclevel-1"> <div style="text-indent:10px;"><a href="#week3#1"><span class="tocnumber"></span> <span class="toctext">#1 Selection / Swimming</span></a></li>
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<h2><b><a name="week3#2">#2 Speed Improvement - 3rd week</a></b></h2>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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<h2><b>V05_17 </b></h2><br>
 +
<b>Preparation of overnight cultures</b><br>
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<ul>
 +
<li>Experiment: <br> Over night cultures for the preparation of chemically competent cells were prepared using <i>E. coli</i> (DH10B) cells.
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<h2><b>V05_18 </b></h2><br>
<h2><b>V05_18 </b></h2><br>
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<b>V05_18_1 Double Digest of FlhDC and puc18</b><br>
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<b>V05_18_1 Double Digestion of <i>flhDC</i> and pUC18</b><br>
<ul>
<ul>
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<li>Experiment: Both parts were digested with EcoRI and Pst1</li>
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<li>Experiment: <br>
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<li>Description: <br> </li>
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Both parts were digested with <i>EcoRI</i> and <i>PstI</i> using Orange Buffer. </li>
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<li>Observations & Results: <br> </li>
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<br>
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<b>V05_18_2 Preparing competent cells of <i> E.coli</i> (DH10B)</b><br>
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<b>V05_18_2 Preparing chemically competent cells of <i> E.coli</i> (DH10B)</b><br>
<ul>
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<li>Experiment: </li>
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<li>Experiment: <br>
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The preparation of the competent cells was performed according to the following <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.</li>
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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<b>Important pages</b>:<br>
 
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<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
 
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<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
 
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Latest revision as of 17:17, 22 September 2012

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#2 Speed Improvement - 3rd week

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V05_17


Preparation of overnight cultures
  • Experiment:
    Over night cultures for the preparation of chemically competent cells were prepared using E. coli (DH10B) cells.



V05_18


V05_18_1 Double Digestion of flhDC and pUC18
  • Experiment:
    Both parts were digested with EcoRI and PstI using Orange Buffer.

V05_18_2 Preparing chemically competent cells of E.coli (DH10B)
  • Experiment:
    The preparation of the competent cells was performed according to the following protocol.


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