Team:Goettingen/week2-2

From 2012.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
-
{{GoettingenHeader}}
+
{{GoettingenHeader|deu=Team:Goettingen/week2|eng=Team:Goettingen/week2}}
 +
<html>
 +
<table>
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en">
 
-
<head>
 
-
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
 
-
<meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
 
-
<link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
 
-
<link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
 
-
<link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&amp;action=creativecommons" rel="meta">
 
-
<link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=rss">
 
-
<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=atom">
 
-
</head>
 
-
<body class="mediawiki ns-0 ltr page-Team_Goettingen">
 
-
 
-
<font face="Verdana" size="-1">Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English</a> <br>
 
Line 66: Line 55:
<ul>
<ul>
<li>Experiment: <br>  The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual. </li>
<li>Experiment: <br>  The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual. </li>
-
<br> </li>
 
</ul>
</ul>
<br></td></tr>
<br></td></tr>
</table>
</table>
-
<br>
+
 
-
<body id="top">
+
 
-
<a href="#top">&uarr; Return to top</a>
+
 
-
<br>
+
<br>
<br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<br>
<br>
</td>
</td>
-
 
-
 
-
<table bordercolor="black" border="1 px" width="600 px"><tr><td>
 
-
<b>Important pages</b>:<br>
 
-
<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
 
-
<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
 
-
</td></tr>
 
-
</table>
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
                                 <!-- Text body end-->
                                 <!-- Text body end-->
Line 108: Line 72:
</body>
</body>
</html>
</html>
 +
{{GoettingenFooter}}

Latest revision as of 17:17, 22 September 2012

Deutsch  / English 

#2 Speed Improvement - 2nd week

Back to overview

V05_08


Amplification of flhDC gene
  • Experiment:
    Using the isolated genomic DNA of E. coli (DH10B) as template, the gene flhDC was amplified by pfu polymerase according to the following protocol.



V05_10


Separation of flhDC
  • Experiment:
    The amplicon was analysed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
  • Observations & Results:
    The gel featured a clear band at about 900 kb. The amplification of flhDC was successful.


V05_11


Purificaton of flhDC
  • Experiment:
    The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.


Back to overview

↑ Back to top!