Team:Goettingen/week2-2

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#2 Speed Improvement - 2nd week

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V05_08

Amplification of flhDC gene

Experiment:
Using the isolated genomic DNA of E. coli (DH10B) as template, the gene flhDC was amplified by pfu polymerase according to the following protocol.

V05_10

Separation of flhDC

Experiment:
The amplicon was analysed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
Observations & Results:
The gel featured a clear band at about 900 kb. The amplification of flhDC was successful.

V05_11

Purificaton of flhDC

Experiment:
The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.

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-                        <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Chemotaxis"><span><span><div style="text-indent:20px;">&#8901; Chemotaxis</div></span></span></a></li>  -->
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-                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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-                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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-                                <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Summary"><span><span>Summary</span></span></a></li>
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-                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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-                                <!--<li><a href="https://2012.igem.org/Team:Goettingen/BioBrick_System"><span><span>iGEM BioBrick System</span></span></a></li>
-                                <li><a href="https://2012.igem.org/Team:Goettingen/Part_Registry"><span><span>iGEM Part Registry</span></span></a></li>  -->
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-                </li>
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-                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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-                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen"><span><span>City</span></span></a></li>
-                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/University"><span><span>Georg-August-University</span></span></a></li>
-                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/MPI"><span><span>Max-Planck-Institute</span></span></a></li>
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-                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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-                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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-                <li style="width: 160px">
-                        <a href="https://2012.igem.org/Team:Goettingen/Human_Practice" style="color: white;">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
-                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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-                               <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Public_and_Media"><span><span>Public and Media</span></span></a></li>
-                               <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">&#8901; Newspaper</div></span></span></a></li>
-                               </li><li><a href="https://2012.igem.org/Team:Goettingen/Conference"><span><span><div style="text-indent:20px;">&#8901; Conference</div></span></span></a></li>
-                               </li><li><a href="https://2012.igem.org/Team:Goettingen/Synthetic_Biology_Day"><span><span><div style="text-indent:20px;">&#8901; Synthetic Biology Day</div></span></span></a></li> -->
-                               <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Survey"><span><span>Survey</span></span></a></li>
-                               <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion"><span><span>Panel Discussion</span></span></a></li>-->
-                               <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli"><span><span>Flash Coli</span></span></a></li>
-                               </ul>
-                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
-                </li>
-                <li>
-                        <a href="https://2012.igem.org/Team:Goettingen/Sponsoring" style="color: white;">Sponsoring</a>
-                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
-                                <ul class="DropDownMenu" id="MB1-DDM2-SM3">
-                               <li><a href="https://2012.igem.org/Team:Goettingen/Sponsoring"><span><span>Interested in...?</span></span></a></li>
-                                <!-- <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Sponsors"><span><span>Sponsors</span></span></a></li>
-                                <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Supporters"><span><span>Supporters</span></span></a></li>     -->
-                </li>
-        </ul>
-</div>
-<table>
-                                 <!-- Text body -->
-<tbody><tr valign="top" align="left">
-<td style="padding: 0pt 20px 0pt 0pt;" width="650px">
-<font face="Verdana" size="-1">
-<br>
-Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
-<br>
@@ Line 538: / Line 28: @@
 <b>Amplification of <i>flhDC</i> gene</b><br>
 <ul>
-<li>Experiment: <br> Using the isolated genomic DNA of DH10B as template, the gene <i>flhDC</i> was amplified by <i>pfu</i> polymerase according to the following <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.</li>
+<li>Experiment: <br> Using the isolated genomic DNA of <i>E. coli</i> (DH10B) as template, the gene <i>flhDC</i> was amplified by <i>pfu</i> polymerase according to the following <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.</li>
 <br> </li>
 </ul>
@@ Line 565: / Line 55: @@
 <ul>
 <li>Experiment: <br>  The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual. </li>
-<br> </li>
 </ul>
 <br></td></tr>
 </table>
-<br>
-<body id="top">
-<a href="#top">&uarr; Return to top</a>
-<br>
 <br>
 <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
 <br>
 </td>
-<table bordercolor="black" border="1 px" width="600 px"><tr><td>
-<b>Important pages</b>:<br>
-<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
-<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
-<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
-<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
-<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
-<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
-<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
-<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
-<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
-</td></tr>
-</table>
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@@ Line 607: / Line 72: @@
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+{{GoettingenFooter}}