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Team:TMU-Tokyo/Notebook/Protocol/
From 2012.igem.org
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</p> | </p> | ||
| - | <p class=" | + | <p class="description"> |
<b>■Plasmid DNA Purification</b><Br> | <b>■Plasmid DNA Purification</b><Br> | ||
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<Br> | <Br> | ||
<b>■Genome DNA Purification</b>; Generation™ Capture Column (QIAGEN)<Br> | <b>■Genome DNA Purification</b>; Generation™ Capture Column (QIAGEN)<Br> | ||
| - | 1. Add 200µL overnight cultures of E. coli in LB medium to the Capture Column | + | <Br> |
| - | touching the center of the matrix with the pipet tip | + | <b>1.</b> Add 200µL overnight cultures of E. coli in LB medium to the Capture Column, by gently touching the center of the matrix with the pipet tip.<Br> |
| - | + | <b>2.</b> Incubate 1 min at room temperature.<Br> | |
| + | <b>3.</b> Add 400µL DNA Purification Solution 1, incubate 1 min.<Br> | ||
| + | <b>4.</b> Centrifuge 10 s with tabletop centrifuge.<Br> | ||
| + | <b>5.</b> Transfer the Capture Column to the Waste Collection Tube.<Br> | ||
| + | |||
| + | |||
<Br> | <Br> | ||
Revision as of 17:37, 21 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
Plasmid DNA Purification
Genome DNA Purification
Restruction Enzyme Degestion
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Protocol
■Plasmid DNA Purification
■Genome DNA Purification; Generation™ Capture Column (QIAGEN)
1. Add 200µL overnight cultures of E. coli in LB medium to the Capture Column, by gently touching the center of the matrix with the pipet tip.
2. Incubate 1 min at room temperature.
3. Add 400µL DNA Purification Solution 1, incubate 1 min.
4. Centrifuge 10 s with tabletop centrifuge.
5. Transfer the Capture Column to the Waste Collection Tube.
■Restruction Enzyme Degestion
■DNA Fragment Ligation
■Transformation
■Electrophoresis
■LB Medium
■PCR
