"
Team:Bielefeld-Germany/Labjournal/week21
From 2012.igem.org
(Difference between revisions)
(→Wednesday September 19th) |
(→Wednesday September 19th) |
||
| Line 9: | Line 9: | ||
===Wednesday September 19th=== | ===Wednesday September 19th=== | ||
* '''Team Cellulose Binding Domain:'''Stopped over-night-''Dpn''I-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar. | * '''Team Cellulose Binding Domain:'''Stopped over-night-''Dpn''I-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar. | ||
| - | **Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced)with the CBDcex_Freiburg | + | **Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced) with the CBDcex_Freiburg and GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures from 57°C to 64°C; merged the correct fractions and cleaned them up through the gel. |
| - | **Digested the cleaned up product with ''Spe''I and ''Xba''I and ''Dpn''I | + | **Digested the cleaned up product with ''Spe''I and ''Xba''I and ''Dpn''I. |
===Thursday September 20th=== | ===Thursday September 20th=== | ||
Revision as of 16:10, 19 September 2012
Contents |
Week 21 (09/17 - 09/23/12)
Monday September 17th
Tuesday September 18th
Wednesday September 19th
- Team Cellulose Binding Domain:Stopped over-night-DpnI-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar.
- Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced) with the CBDcex_Freiburg and GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures from 57°C to 64°C; merged the correct fractions and cleaned them up through the gel.
- Digested the cleaned up product with SpeI and XbaI and DpnI.
Thursday September 20th
Friday September 21st
Saturday September 22nd
Sunday September 23rd
Sunday
| 55px |  |  |  |  |  |  |  |  | 
| 
|
