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Team:Bielefeld-Germany/Labjournal/week21
From 2012.igem.org
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| + | * '''Team Cellulose Binding Domain:'''Stopped over-night-''Dpn''I-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar. | ||
| + | **Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced)with the CBDcex_Freiburg+GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures form 57°C to 64°C; merged the products and cleaned them through gel and clean-up kit. | ||
| + | **Digested the cleaned up product with ''Spe''I and ''Xba''I and ''Dpn''I | ||
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===Thursday September 20th=== | ===Thursday September 20th=== | ||
===Friday September 21st=== | ===Friday September 21st=== | ||
Revision as of 16:08, 19 September 2012
Contents |
Week 21 (09/17 - 09/23/12)
Monday September 17th
Tuesday September 18th
Wednesday September 19th
- Team Cellulose Binding Domain:Stopped over-night-DpnI-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar.
- Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced)with the CBDcex_Freiburg+GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures form 57°C to 64°C; merged the products and cleaned them through gel and clean-up kit.
- Digested the cleaned up product with SpeI and XbaI and DpnI
Thursday September 20th
Friday September 21st
Saturday September 22nd
Sunday September 23rd
Sunday
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