"
Team:Bielefeld-Germany/Labjournal/week10
From 2012.igem.org
(→Wednesday July 4th) |
(→Wednesday July 4th) |
||
| Line 12: | Line 12: | ||
* '''Team Bacterial Laccases:''' | * '''Team Bacterial Laccases:''' | ||
| - | After all failed attempts to isolate the laccase genes from ''Streptomyces lavendulae'' and ''Streptomyces griseus'' we assumed that maybe the sequences from this laccases and the laccase sequences we designed the primers for were to different to get a product [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th look here for more details]. So we used the sequences of the laccase genes we tried to isolate from the ''Streptomycetes'' strains and blasted them against an intern database from our university. The results showed that we could use genomic DNA from ''Streptomyces sp. tuebingen'', ''S.roseochromogenes'' and ''Streptomyces sp. goettingen'', from which we can get already isolated genomic DNA. Therefore we designed primers for the isolation of the predicted laccase genes from these three strains. | + | After all failed attempts to isolate the laccase genes from ''Streptomyces lavendulae'' and ''Streptomyces griseus'' we assumed that maybe the sequences from this laccases and the laccase sequences we designed the primers for were to different to get a product [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th (look here for more details)]. So we used the sequences of the laccase genes we tried to isolate from the ''Streptomycetes'' strains and blasted them against an intern database from our university. The results showed that we could use genomic DNA from ''Streptomyces sp. tuebingen'', ''S.roseochromogenes'' and ''Streptomyces sp. goettingen'', from which we can get already isolated genomic DNA. Therefore we designed primers for the isolation of the predicted laccase genes from these three strains. |
===Thursday July 5th=== | ===Thursday July 5th=== | ||
Revision as of 15:02, 15 September 2012
Contents |
Week 10 (07/02 - 07/08/12)
Monday July 2nd
Team Fungal Laccases: Sending a request for plasmids containing cDNA sequences of five different laccases from Trametes versicolor and Pycnoporus cinnabarinus from the Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis at Ernst-Moritz-Arndt-University in Greifswald.
Tuesday July 3rd
Wednesday July 4th
- Team Student Academy: Final meeting with all instructors of the student academy to talk about all details.
- Team Bacterial Laccases:
After all failed attempts to isolate the laccase genes from Streptomyces lavendulae and Streptomyces griseus we assumed that maybe the sequences from this laccases and the laccase sequences we designed the primers for were to different to get a product (look here for more details). So we used the sequences of the laccase genes we tried to isolate from the Streptomycetes strains and blasted them against an intern database from our university. The results showed that we could use genomic DNA from Streptomyces sp. tuebingen, S.roseochromogenes and Streptomyces sp. goettingen, from which we can get already isolated genomic DNA. Therefore we designed primers for the isolation of the predicted laccase genes from these three strains.
Thursday July 5th
- Team shuttle vector: Some fragments for Gibson Assembly with overlapping DNA sequences were amplified via Phusion-PCR. The fragment 5'AOX1 was amplified with the primer pair pSB1C3-5aox1-f and pSB1C3-5aox1-r. The fragment MF-alpha1 was amplified with the primer pair 5aox1-mfalpha1-f and 5aox1-mfalpha1-r. The fragment 3'AOX1 was amplified with the primer pair his4-3aox1-f and his4-3aox1-r. The fragment pSB1C3 was amplified with the primer pair 3aox1-pSB1C3-f and 3aox1-pSB1C3-r.
Friday July 6th
- Team Student Academy: The two presentations were prepared and the barbecue for the pupils was organized. In the lab the last preparations were made.
Saturday July 7th
Sunday July 8th
Sunday
| 55px |  |  |  |  |  |  |  |  | 
| 
|
