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Team:Bielefeld-Germany/Labjournal/week10
From 2012.igem.org
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===Thursday July 5th=== | ===Thursday July 5th=== | ||
| - | * '''Team shuttle vector:''' Some fragments for Gibson Assembly with overlapping DNA sequences were amplified via Phusion-PCR. The fragment 5'AOX1 was amplified with the | + | * '''Team shuttle vector:''' Some fragments for Gibson Assembly with overlapping DNA sequences were amplified via Phusion-PCR. The fragment 5'AOX1 was amplified with the primer pair pSB1C3-5aox1-f and pSB1C3-5aox1-r. The fragment MF-alpha1 was amplified with the primer pair 5aox1-mfalpha1-f and 5aox1-mfalpha1-r. The fragment 3'AOX1 was amplified with the primer pair his4-3aox1-f and his4-3aox1-r. The fragment pSB1C3 was amplified with the primer pair 3aox1-pSB1C3-f and 3aox1-pSB1C3-r. |
Revision as of 02:21, 14 September 2012
Contents |
Week 10 (07/02 - 07/08/12)
Monday July 2nd
- Sending a request for plasmids containing cDNA sequences of five different laccases from Trametes versicolor and Pycnoporus cinnabarinus from the Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis at Ernst-Moritz-Arndt-University in Greifswald.
Tuesday July 3rd
Wednesday July 4th
- Team Student Academy: Final meeting with all instructors of the student academy to talk about all details.
Thursday July 5th
- Team shuttle vector: Some fragments for Gibson Assembly with overlapping DNA sequences were amplified via Phusion-PCR. The fragment 5'AOX1 was amplified with the primer pair pSB1C3-5aox1-f and pSB1C3-5aox1-r. The fragment MF-alpha1 was amplified with the primer pair 5aox1-mfalpha1-f and 5aox1-mfalpha1-r. The fragment 3'AOX1 was amplified with the primer pair his4-3aox1-f and his4-3aox1-r. The fragment pSB1C3 was amplified with the primer pair 3aox1-pSB1C3-f and 3aox1-pSB1C3-r.
Friday July 6th
- Team Student Academy: The two presentations were prepared and the barbecue for the pupils was organized. In the lab the last preparations were made.
Saturday July 7th
Sunday July 8th
Sunday
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