Team:TMU-Tokyo/Project

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We planed to create E.coli which removes such a hazardous material, named <Strong>Chef Ant E.coli</Strong>. It is appended 3 devices and gets removal ability of formaldehyde.
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We planed to create E.coli which removes such a hazardous material, named <Strong>Chef Ant E.coli</Strong>. It is appended 3 devices and gets removal ability of formaldehyde.<Br>
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<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device1"><b>Device1</b></A>; Visualize formaldehyde with GFP.<Br>
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<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device2"><b>Device2</b></A>; Dehydrogenize formaldehyde to formate.<Br>
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<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device3"><b>Device3</b></A>; Dehydrogenize formate to carbon dioxide and water.<Br>
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Details of our devices is available each device page.<Br>
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1. Tonegawa's formaldehyde levels spike ― <A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">DAILY YOMIURI ONLINE</A><Br><Br><Br><Br><Br>
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fig.1<Br>
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This is the pathway of formaldehyde visualization and removal.
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Device1; <p class="description"><Strong>Sensing formaldehyde and then producing GFP</Strong><Br>
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In <i>frmRAB</i> operon, which origin is chromosome of <i>Escherichia coli</i>, <i>frmAB</i> is normally repressed by frmR. But, when formaldehyde exists, <i>frmR</i> loses its inhibitory(2). This device is composed of <i>frmR</i> gene, its promoter and GFP gene.</p><Br>
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2. <Strong>Christopher D. Herring and Frederick R. Blattner.</Strong> 2004. Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator <i>frmR</i>[<A Href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC522192/">PubMed</A>]</p>
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1. Tonegawa's formaldehyde levels spike ― <A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">DAILY YOMIURI ONLINE</A><Br><Br><Br><Br><Br><Br>
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Device2; <p class="description"><Strong>Producing formaldehyde dehydrogenase, changing formaldehyde to formate</Strong><Br>
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<i>Escherichia coli</i> has original formaldehyde dehydrogenase, but it depends on glutathione (existence). Instead of it, we found glutathione-independent formaldehyde dehydrogenase in <i>Pseudomonas putida</i>. This device is composed of its gene and constitutive promoter.<Br></p>
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fig.1 This is the pathway of formaldehyde visualization and removal.
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Device3; <p class="description"><Strong>Producing formate dehydrogenase, changing formate to water and carbon dioxide</Strong><Br>
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This formate dehydrogenase gene is derived from <i>Methylobacterium extorquens</i>. As formaldehyde dehydrogenase, <i>Escherichia coli</i> has similar function gene of this gene. But it has too long length to insert high copy plasmid.
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<i>Escherichia coli</i> has a gene that has the similar function as a formate dehydrogenase but it is too long to be inserted into the high copy plasmid. Therefore, we used the <i>Methylobacterium extorquens</i>’s one.
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This device is composed of its gene and constitutive promoter.</p><Br>
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Revision as of 15:54, 8 September 2012

 


Abstract


 
 

Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.(1)



We planed to create E.coli which removes such a hazardous material, named Chef Ant E.coli. It is appended 3 devices and gets removal ability of formaldehyde.

Device1; Visualize formaldehyde with GFP.
Device2; Dehydrogenize formaldehyde to formate.
Device3; Dehydrogenize formate to carbon dioxide and water.

Details of our devices is available each device page.



1. Tonegawa's formaldehyde levels spike ― DAILY YOMIURI ONLINE





fig.1 This is the pathway of formaldehyde visualization and removal.

 

Retrieved from "http://2012.igem.org/Team:TMU-Tokyo/Project"