Team:UT-Tokyo

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iGEM UT-Tokyo aim to improve Escherichia coli's intrinsic hydrogen synthesis pathway by transgenics and to make E.coli produces hydrogen more efficiently with a view of using it as an energy source.

Sweetaholic Energy Generator:
Hydrogen Production from Sugar-rich Waste by E.coli

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　Today, large quantities of food and drinks, together with the enormous amounts of energy they contain, are dumped without being reused. However, the moisture in this waste prevents it from being used as a energy source by burning. Our project aims to reuse such nutritious garbage by Escherichia coli digesting glucose to synthesize hydrogen, which is expected to be used in various useful ways, such as in fuel cells.


E. coli cells have intrinsic metabolic systems related to synthesis of hydrogen from glucose via formic acid. We are trying to improve the latter part of this metabolic system, the formic acid-hydrogen pathway in two ways. One is by overexpressing a transcriptional gene which enhances/ a step in this pathway, and the other is by repressing the inhibitive gene of the hydrogen production. If we are successful, what we have to do for getting energy is to share our sweets and juices with E. coli.



Team
About Our Team
Project1: H2 E.coli
H2 Production
Project2: Inhibition without Knockout
Inhibit Transcription Factors without Knockout
Parts
The BioBrick parts we made
Lab Work
Protocols & Lab Note & Safety
Human Practice
Book Review & Campus Festival

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