Question 1
1.Would any of your project ideas raise safety issues in terms of:researcher safety,
public safety, or
environmental safety?
The bacteria upon which we have being operating is non-pathogenic E.coli epi300,an organism at BSL-1 according to standards of CDC in the United States. Minor risks are contained in our research since the organism and plasmids, which causes no severe infection to human and animals, will be handled properly by well-trained personnel after every and each experiment, and the chemicals in frequent use are proved to be friendly, except EB ,which is separated along with related devices in a special section, the contact with which requires such protections as a pair of rubber gloves. Prior to the researches of the project, our team members received all basic trainings on biosafety and laboratory safety in turn ,lectured by professors in the laboratory and participants in 2011 iGEM on Tsinghua-A team. Thus no major safety problem will possibly be encountered, which will be under control if they would emerge.