Team:NTNU Trondheim/links

From 2012.igem.org

NTNU IS B.A.C.K.
Bacterial Anti-Cancer-Kamikaze

Links

This page contains links related to both our project, iGEM and molecular biology in general:

Contents


BioBrick parts and genes of interest

Escherichia coli lct operon ([1],[2], [3]) (source of lactate inducible promoter system)

<partinfo>Bba_K112808</partinfo> - Enterobacteria phage T4 Lysis Device - no promoter.

iGEM/BioBricks resources and information

Lecture videos

Standard Assembly

Restriction enzymes and the BioBrick system

BioBrick prefix and suffix

Assembly strategies

3A Assembly

Protein coding

Ribosome Binding Site

Design of Ribosome Binding Sites

Distribution kits - about

Parts registry help page

Antibiotic resistance of plasmid backbones

DNA Assembly

DNA

Plasmid backbones nomenclature

Spring 2012 DNA distribution kit contents

BioBrick Assembly kit sold by New England BioLabs.

BioBricks Assembly kit manual.

Open WetWare: parts fabrication

Genomics and bioinformatics resources

http://www.ecogene.org/ - Updated information about the E. coli K-12 genome and proteome sequences

E. coli links

E coli DH1, complete genome

Escherichia coli str. K-12 substr. MG1655, complete genome

Glimmer (gene finder) and RBSfinder

Promoter construction

E. coli genotypes]

Bionumers - The database of biological numbers

Using the Registry

Quality Control

How to check a part

Sequence analysis instructions

Reading gel images

Molecular biology

AHL. Parts registry page on [N-]acyl-homoserine lactones, molecules used in cell signalling.

Lux Parts registry page on the Lux genetic regulatory system.

Similar and relevant projects

Brigham Young University iGEM 2012: http://openwetware.org/wiki/BYU_iGEM_2012 (Cancer detection, Lactacte sensor)

"Engineering microbes to sense and eradicate Pseudomonas Aeruginosa, a human pathogen" Mol Syst Biol. 2011 Aug 16;7:521

http://www.ncbi.nlm.nih.gov/pubmed/16330045

https://2008.igem.org/Team:NTU-Singapore

Software

Cain: Stochastic Simulations for Chemical Kinetics

J5 Automated DNA Assembly Software

NEBCutter 2

WatCut: An on-line tool for restriction analysis, silent mutation scanning, and SNP-RFLP analysis

Just for fun

"The PCR song": http://www.youtube.com/watch?v=x5yPkxCLads

"Protein synthesis: an epic on the cellular level": http://www.youtube.com/watch?v=u9dhO0iCLww

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