Team:NTNU Trondheim/links

From 2012.igem.org

NTNU IS B.A.C.K.
Bacterial Anti-Cancer-Kamikaze

Links

This page contains links related to both our project, iGEM and molecular biology in general:

Contents


BioBrick parts and genes of interest

Escherichia coli lct operon ([http://www.ncbi.nlm.nih.gov/nuccore/L13970],[http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU164], [http://www.ecogene.org/geneInfo.php?eg_id=EG11962&sites=BamHI,%20EcoRI,%20HindIII]) (source of lactate inducible promoter system)

<partinfo>Bba_K112808</partinfo> - Enterobacteria phage T4 Lysis Device - no promoter.

iGEM/BioBricks resources and information

Lecture videos

[http://partsregistry.org/Assembly:Standard_assembly Standard Assembly]

[http://partsregistry.org/Assembly:Restriction_enzymes Restriction enzymes and the BioBrick system]

[http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix BioBrick prefix and suffix]

[http://partsregistry.org/Assembly:Rolling_assembly Assembly strategies]

[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/3A_assembly 3A Assembly]

[http://partsregistry.org/Help:Protein_coding Protein coding]

[http://partsregistry.org/Help:Ribosome_Binding_Site Ribosome Binding Site]

[http://partsregistry.org/Ribosome_Binding_Sites/Design Design of Ribosome Binding Sites]

[http://partsregistry.org/Help:Distribution_Kits Distribution kits - about]

[http://partsregistry.org/Help:Contents Parts registry help page]

[http://partsregistry.org/Antibiotic_Resistance Antibiotic resistance of plasmid backbones]

[http://partsregistry.org/DNA/Assembly DNA Assembly]

[http://partsregistry.org/DNA DNA]

[http://partsregistry.org/wiki/index.php/Help:Plasmids/Nomenclature Plasmid backbones nomenclature]

[http://partsregistry.org/assembly/libraries.cgi?id=42 Spring 2012 DNA distribution kit contents]

[http://www.neb.com/nebecomm/products/productE0546.asp BioBrick Assembly kit] sold by New England BioLabs.

[http://ginkgobioworks.com/support/ BioBricks Assembly kit manual].

[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication Open WetWare: parts fabrication]

Genomics and bioinformatics resources

http://www.ecogene.org/ - Updated information about the E. coli K-12 genome and proteome sequences

[http://cgsc.biology.yale.edu/ColiLinks.php E. coli links]

[http://www.ncbi.nlm.nih.gov/bioproject/162051 E coli DH1, complete genome]

[http://www.ncbi.nlm.nih.gov/nuccore/U00096.2 Escherichia coli str. K-12 substr. MG1655, complete genome]

[http://nbc11.biologie.uni-kl.de/framed/left/menu/auto/right/glimmer2.02/ Glimmer (gene finder) and RBSfinder]

[http://partsregistry.org/Help:Promoters/Construction Promoter construction]

[http://openwetware.org/wiki/E._coli_genotypes E. coli genotypes]]

[http://bionumbers.hms.harvard.edu/default.aspx Bionumers - The database of biological numbers]

Using the Registry

[http://partsregistry.org/Help:Quality_Control Quality Control]

[http://partsregistry.org/Help:How_to_check_a_part How to check a part]

[http://partsregistry.org/Help:Sequence_Analysis_Instructions Sequence analysis instructions]

[http://partsregistry.org/Help:Reading_Gel_Images Reading gel images]

Molecular biology

[http://partsregistry.org/AHL AHL]. Parts registry page on [N-]acyl-homoserine lactones, molecules used in cell signalling.

[http://partsregistry.org/Lux Lux] Parts registry page on the Lux genetic regulatory system.

Similar and relevant projects

Brigham Young University iGEM 2012: http://openwetware.org/wiki/BYU_iGEM_2012 (Cancer detection, Lactacte sensor)

[http://www.ncbi.nlm.nih.gov/pubmed/21847113 "Engineering microbes to sense and eradicate Pseudomonas Aeruginosa, a human pathogen" Mol Syst Biol. 2011 Aug 16;7:521]

http://www.ncbi.nlm.nih.gov/pubmed/16330045

https://2008.igem.org/Team:NTU-Singapore

Software

[http://cain.sourceforge.net/ Cain: Stochastic Simulations for Chemical Kinetics]

[http://j5.jbei.org/index.php/Main_Page J5 Automated DNA Assembly Software]

[http://tools.neb.com/NEBcutter2/ NEBCutter 2]

[http://watcut.uwaterloo.ca/watcut/watcut/template.php WatCut: An on-line tool for restriction analysis, silent mutation scanning, and SNP-RFLP analysis]

Just for fun

"The PCR song": http://www.youtube.com/watch?v=x5yPkxCLads

"Protein synthesis: an epic on the cellular level": http://www.youtube.com/watch?v=u9dhO0iCLww

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