Team:UT-Tokyo/Notebook

From 2012.igem.org

(Difference between revisions)
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*Miniprep
*Miniprep
** #3, #5, #20
** #3, #5, #20
 +
 +
==== July 13th ====
 +
*Nanodrop (#6).
 +
*Digestion (#3:E-P, #5:E-P, #20:E-P)
 +
*Electrophoresis (#3, #5, #20)
 +
 +
==== July 14th ====
 +
*Miniprep (#1,3,5,11,20)
 +
*Gel extraction (#6)
<html></p>
<html></p>
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<div id="Week08" class="Weeks">
<div id="Week08" class="Weeks">
<p></html>
<p></html>
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==== July 17th ====
 +
*Transformation (#1A2,#3,#5,#20)
 +
*PCR (parts of 1. and #3,#20(Colony PCR))
 +
*Incubate #5.
 +
 +
==== July 18th ====
 +
*Miniprep (#1A2,#3,#5,#20)
 +
*PCR using u.p (#1A2,#3,#5,#20)
 +
*Colony PCR (#3,#20,H₂O)
 +
*Electrophoresis (#1A2,#3,#5,#20, #3,#20, H₂O)
 +
*PCR (2011’s #1,#36 and 2012’s #3,#5,#20, H₂O)
 +
 +
==== July 20th ====
 +
*Miniprep.
 +
*Electrophoresis (7/19’s PCR product(#1,#3,#5,#20,#36) )
 +
*Colony PCR (#3,#5,#20)
 +
 +
==== July 21st ====
 +
*Electrophoresis (7/20’s PCR product (#3,#5,#20))
 +
<html></p>
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<div id="Week09" class="Weeks">
<div id="Week09" class="Weeks">
<p></html>
<p></html>
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==== July 24th ====
 +
*Making agar medium plate contain ampicillin
 +
*PCR (#6, #8)
 +
*Electrophoresis (#6, #8)
 +
*Transformation (#1)
 +
*Culture (#5, #20)
 +
 +
==== July 25th ====
 +
*Miniprep (#5, #20)
 +
*Purify (#6, #8)
 +
*Digestion (#3:S-P,#5:E-X,#11:E-S,#20:S-P)
 +
*Electrophoresis (#5, #11)
 +
*Ligation (#5,#11) (?)
 +
 +
==== July 26th ====
 +
*Culture (#1)
 +
*Digestion (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
 +
*Electrophoresis (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
 +
*Gel extraction (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
 +
 +
==== July 27th ====
 +
*Culture, Miniprep (#1)
 +
*Digestion (#5-A:E-X, #11-A:X-P)
 +
*Electrophoresis (#3:S-P, #20:S-P, #11:E-S)
 +
*Gel extraction (#3:S-P, #20:S-P, #11:E-S, E-S vector, hycAp:E-S)
 +
 +
==== July 28th ====
 +
ごめんなさい、何やったのかノートからではよく読み取れませんでした;;th ====
 +
<html></p>
<html></p>
</div>
</div>
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 +
<h3 onclick="ShowNote('Week10');" onkeypress="ShowNote('Week10');">10th week: 7/29-8/4</h3>
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<div id="Week10" class="Weeks">
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<p></html>
 +
 +
==== July 30th ====
 +
*Digestion (#3:S-P, #11:X-P)
 +
*Electrophoresis (#3, #11)
 +
*Gel extraction (#3, #11)
 +
*Ligation (#3, #11)
 +
*Transformation (ligation product; #3-11)
 +
 +
==== August 1th ====
 +
*Digestion (#3:S-P, #11:X-P)
 +
*Electrophoresis (#3, #11)
 +
*Gel extraction (#3, #11)
 +
*Ligation (#3, #11)
 +
*Transformation (ligation product; #3-11)
 +
*Miniprep (#3, #5, #11)
 +
 +
==== August 2th ====
 +
*Colony PCR (#3-11)
 +
*Culture (#3-11)
 +
*Colony pick up, culture (red colony of #11)
 +
*Digestion (#3:S-P, #5:E-X)
 +
 +
==== August 3th ====
 +
*Gel extraction (#3:S-P, #5:E-X)
 +
*Making solution of Kanamycin (100mg/mL)
 +
*Culture, miniprep (#11)
 +
*Culture (#11)
 +
 +
 +
 +
<html></p>
 +
</div>
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<h3 onclick="ShowNote('Week11');" onkeypress="ShowNote('Week11');">11th week: 7/29-8/4</h3>
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<div id="Week11" class="Weeks">
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<p></html>
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 +
<html></p>
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</div>
</div>
</div>

Revision as of 10:09, 26 September 2012

Notebook

box-background image

このページの概要を、簡単に記述。


May

1st week: 5/27-6/2

May 29th

  • Making LB medium

May 30th

  • Making LB Agar
  • Making LB Amp Plates


May 31st

  • Preculture


June

2nd week: 6/3-6/9

June 5th

  • Plating JM109

June 6th

  • preculture

June 7th

  • Measuring OD by spectroscope
  • Meking electrocompetent cells
  • Electroporating of a test plasmid

June 8th

  • Caluculatiing transformation efficiency


3rd week: 6/10-6/16

4th week: 6/17-6/23

5th week: 6/24-6/30

June 26th

  • Making LB tetracycline plate

June 27th

June 28th

  • Making LB kanamycin plate and chloramphenicol plate
  • Transformation with a test plasmid

June 29th

  • Transformation with #1(constitutive promoter) and #11(RBS)

2012/07

6th week: 7/1-7/7

July 3rd

  • Making K solution
  • Colony PCR DNA fragment assumed as fhlA (#6) for cloning

July 4th

  • Miniprep #1, #11

July 5th

  • Making 1% agarose solution
  • Electrophoresis (#6)
  • Digest (#1_EP, #11-_EP)

July 6th

  • Colony PCR (#6)

7th week: 7/8-7/14

July 9th

  • Electrophoresis (#6)

July 10th

  • Transformation
    • #3(RBS medium), #5(d.term), #20(pLac)

July 11th

  • Preculture
    • #3, #5, #20
  • Gel Extraction(#6)

July 12th

  • The continuation of Gel Extraction(#6)
  • Miniprep
    • #3, #5, #20

July 13th

  • Nanodrop (#6).
  • Digestion (#3:E-P, #5:E-P, #20:E-P)
  • Electrophoresis (#3, #5, #20)

July 14th

  • Miniprep (#1,3,5,11,20)
  • Gel extraction (#6)

8th week: 7/15-7/21

July 17th

  • Transformation (#1A2,#3,#5,#20)
  • PCR (parts of 1. and #3,#20(Colony PCR))
  • Incubate #5.

July 18th

  • Miniprep (#1A2,#3,#5,#20)
  • PCR using u.p (#1A2,#3,#5,#20)
  • Colony PCR (#3,#20,H₂O)
  • Electrophoresis (#1A2,#3,#5,#20, #3,#20, H₂O)
  • PCR (2011’s #1,#36 and 2012’s #3,#5,#20, H₂O)

July 20th

  • Miniprep.
  • Electrophoresis (7/19’s PCR product(#1,#3,#5,#20,#36) )
  • Colony PCR (#3,#5,#20)

July 21st

  • Electrophoresis (7/20’s PCR product (#3,#5,#20))


9th week: 7/22-7/28

July 24th

  • Making agar medium plate contain ampicillin
  • PCR (#6, #8)
  • Electrophoresis (#6, #8)
  • Transformation (#1)
  • Culture (#5, #20)

July 25th

  • Miniprep (#5, #20)
  • Purify (#6, #8)
  • Digestion (#3:S-P,#5:E-X,#11:E-S,#20:S-P)
  • Electrophoresis (#5, #11)
  • Ligation (#5,#11) (?)

July 26th

  • Culture (#1)
  • Digestion (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
  • Electrophoresis (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
  • Gel extraction (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)

July 27th

  • Culture, Miniprep (#1)
  • Digestion (#5-A:E-X, #11-A:X-P)
  • Electrophoresis (#3:S-P, #20:S-P, #11:E-S)
  • Gel extraction (#3:S-P, #20:S-P, #11:E-S, E-S vector, hycAp:E-S)

July 28th

ごめんなさい、何やったのかノートからではよく読み取れませんでした;;th ====


10th week: 7/29-8/4

July 30th

  • Digestion (#3:S-P, #11:X-P)
  • Electrophoresis (#3, #11)
  • Gel extraction (#3, #11)
  • Ligation (#3, #11)
  • Transformation (ligation product; #3-11)

August 1th

  • Digestion (#3:S-P, #11:X-P)
  • Electrophoresis (#3, #11)
  • Gel extraction (#3, #11)
  • Ligation (#3, #11)
  • Transformation (ligation product; #3-11)
  • Miniprep (#3, #5, #11)

August 2th

  • Colony PCR (#3-11)
  • Culture (#3-11)
  • Colony pick up, culture (red colony of #11)
  • Digestion (#3:S-P, #5:E-X)

August 3th

  • Gel extraction (#3:S-P, #5:E-X)
  • Making solution of Kanamycin (100mg/mL)
  • Culture, miniprep (#11)
  • Culture (#11)


11th week: 7/29-8/4

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