Team:UT-Tokyo/Notebook

From 2012.igem.org

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==== July 10th ====
==== July 10th ====
*Transformation
*Transformation
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**#3(RBS medium), #5(d.term), #20(pLac)
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** #3(RBS medium), #5(d.term), #20(pLac)
==== July 11th ====
==== July 11th ====

Revision as of 04:28, 26 September 2012

Notebook

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このページの概要を、簡単に記述。


May

1st week: 5/27-6/2

May 29th

  • Making LB medium

May 30th

  • Making LB Agar
  • Making LB Amp Plates


May 31st

  • Preculture


June

2nd week: 6/3-6/9

June 5th

  • Plating JM109

June 6th

  • preculture

June 7th

  • Measuring OD by spectroscope
  • Meking electrocompetent cells
  • Electroporating of a test plasmid

June 8th

  • Caluculatiing transformation efficiency


3rd week: 6/10-6/16

4th week: 6/17-6/23

5th week: 6/24-6/30

June 26th

  • Making LB tetracycline plate

June 27th

June 28th

  • Making LB kanamycin plate and chloramphenicol plate
  • Transformation with a test plasmid

June 29th

  • Transformation with #1(constitutive promoter) and #11(RBS)

2012/07

6th week: 7/1-7/7

July 3rd

  • Making K solution
  • Colony PCR DNA fragment assumed as fhlA (#6) for cloning

July 4th

  • Miniprep #1, #11

July 5th

  • Making 1% agarose solution
  • Electrophoresis (#6)
  • Digest (#1_EP, #11-_EP)

July 6th

  • Colony PCR (#6)

7th week: 7/8-7/14

July 9th

  • Electrophoresis (#6)

July 10th

  • Transformation
    • #3(RBS medium), #5(d.term), #20(pLac)

July 11th

  • Preculture
    • #3, #5, #20
  • Gel Extraction(#6)

July 12th

  • The continuation of Gel Extraction(#6)
  • Miniprep
    • #3, #5, #20

8th week: 7/15-7/21

9th week: 7/22-7/28

This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.


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