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iGEM UT-Tokyo aim to improve Escherichia’s original hydrogen synthesis pathway by transgenics and to make Escherichia produce hydrogen more efficiently.

Hydrogen is expected to be environmental-friendly energy which replaces fossil fuels, which are seriously estimated to be exhausted in several hundred years. However, chemical method of hydrogen synthesis has not been put to practical use, because of costs. On the other hand, use of biological methods of hydrogen production should significantly reduce energy costs, as these processes do not require extensive heating. Escherichia has an ability to produce hydrogen from glucose, as an intrinsic metabolic pathway. However, they produce hydrogen too little to use practically. We are trying to solve this problem by regulating the hydrogen consumption pathway and by enhancing hydrogen synthetic pathway, for Escherichia to produce more hydrogen.

We are going to check the effect of overexpressed fhlA on the activation of FHL and efficiency of hydrogen production by October. In addition, we think of making Escherichia produce hydrogen still more by introducing mutation into fhlA, or by inactivating hycA, which regulates the activity of FhlA.

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