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Team:TU-Delft/part3
From 2012.igem.org
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<p>By combining the olfactory receptor and the <i>FUS1pr-EGFP</> reporter, a complete yeast olfactory system is obtained. If the corresponding ligand binds to the receptor the underlying cascade is turned on and the EGFP is expressed. This EGFP signal can be read out by a fluorescence meter. If the olfactory system will be implemented as a diagnostics tool in developing countries, the EGFP reporter should be changed by a visible reporter. </p><br/> | <p>By combining the olfactory receptor and the <i>FUS1pr-EGFP</> reporter, a complete yeast olfactory system is obtained. If the corresponding ligand binds to the receptor the underlying cascade is turned on and the EGFP is expressed. This EGFP signal can be read out by a fluorescence meter. If the olfactory system will be implemented as a diagnostics tool in developing countries, the EGFP reporter should be changed by a visible reporter. </p><br/> | ||
<h2>Parts</h2> | <h2>Parts</h2> | ||
| - | The receptor constructs and the reporter constructs are combined to have one complete olfactory system. The following biobricks are created: | + | The receptor constructs and the reporter constructs are combined to have one complete olfactory system. The following biobricks are created:<br/> |
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775005 " target="_blank">BBa_K775005</a><br/> | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775005 " target="_blank">BBa_K775005</a><br/> | ||
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775006 " target="_blank">BBa_K775006</a><br/> | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775006 " target="_blank">BBa_K775006</a><br/> | ||
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775007 " target="_blank">BBa_K775007</a><br/> | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775007 " target="_blank">BBa_K775007</a><br/> | ||
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775008 " target="_blank">BBa_K775008</a><br/><br/> | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775008 " target="_blank">BBa_K775008</a><br/><br/> | ||
| - | <h2>Results</h2 | + | <h2>Results</h2> |
<h6>Setup</h6> | <h6>Setup</h6> | ||
With yeast strains transformed with the <i>GPR109A</i> receptor and output <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775005" target="_blank">BBa_K775005</a><br/> and the <i>R17-ODR10<i> receptor and output <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775008 " target="_blank">BBa_K775008</a><br/> a fluorometer experiment was performed. After addition of the ligands OD600 and fluorescence were measured in time. | With yeast strains transformed with the <i>GPR109A</i> receptor and output <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775005" target="_blank">BBa_K775005</a><br/> and the <i>R17-ODR10<i> receptor and output <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K775008 " target="_blank">BBa_K775008</a><br/> a fluorometer experiment was performed. After addition of the ligands OD600 and fluorescence were measured in time. | ||
<h6>Outcome</h6> | <h6>Outcome</h6> | ||
<h6>Conclusions</h6> | <h6>Conclusions</h6> | ||
Revision as of 20:42, 26 September 2012
Introduction
By combining the olfactory receptor and the FUS1pr-EGFP reporter, a complete yeast olfactory system is obtained. If the corresponding ligand binds to the receptor the underlying cascade is turned on and the EGFP is expressed. This EGFP signal can be read out by a fluorescence meter. If the olfactory system will be implemented as a diagnostics tool in developing countries, the EGFP reporter should be changed by a visible reporter.
Parts
The receptor constructs and the reporter constructs are combined to have one complete olfactory system. The following biobricks are created:BBa_K775005
BBa_K775006
BBa_K775007
BBa_K775008
Results
Setup
With yeast strains transformed with the GPR109A receptor and output BBa_K775005and the R17-ODR10 receptor and output BBa_K775008
a fluorometer experiment was performed. After addition of the ligands OD600 and fluorescence were measured in time.
