Single Cell Model

The single-cell pathway model of our system is composed of 4 modules, which are responsible for routing the signal from the receptor to the GFP sythesis. The mathematical model is developed on a scheme favoring the temporal order of processes, based on the current understanding of the pheromone signalling pathway from [1][2][3] and on the feedback received from the experimentalists on the expected behaviour of the pathway, taking into consideration the aspects relevant to our project.

Model of the G - Protein cycle

The concentration of free G-beta-gamma dimer is crucial for the activation of the MAP kinase cascade which in turn activates the expression of the GFP. The promoter used for the receptor and the initial concentration of the ligand were thus crucial parameters for the activation of the G-Protein Cycle. A model of the G-Protein cycle by Yi et al [1] was used to analyze the effects of the different promoter strengths of the receptor and the effects of various initial concentrations on the activation of the G-Protein cycle.