Team:TU-Delft

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<br/><br/><br/>
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<h3><i>Snifferomyces</i>: A Tuberculosis Screening Automaton</h3>
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<p>This year 9 students from TU Delft and 2 students from Leiden along with 7 advisors come together to form team "Snifferomyces" with a goal of providing sniffing capabilities to yeast to achieve rapid and a cost effective diagnosis system for Tuberculosis.
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<br>The 2012 project of the TU Delft iGEM team, draws inspiration from the sniffer rats which can be trained to sniff out unexploded landmines and tuberculosis. Tuberculosis infects around 8 million people a year and kills approximately 2 million. Drugs to treat tuberculosis have been around for a long time, so a rapid diagnosis system can help curb the spread of the disease.</p><br>
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<p style="color: #0900C4;">Collaborations are welcome. Don't hesitate to contact us if you need help on characterizing <br/> your system of fluorescent outputs. We have setups  of flow cytometry, TIRF, <br/> spinning disc confocal microscopy and more.<br/>
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Please contact our Collaboration Manager-Yifan Pan: Y.Pan-2@student.tudelft.nl <p>
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Revision as of 15:32, 13 July 2012

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Snifferomyces

Snifferomyces is a modular system, used in the detection of volatile compounds. It has in the membrane a G-protein–coupled receptor that can bind to a specific signal, once bound it then switches on a signaling machinery which transmits this information over the plasma membrane and through the cell to produce a Quantitative response in the form of fluorescence. Using the Snifferomyces, our aim is to develop a universal olfactory system which allows scientists to introduce olfactory receptors in yeast with minimal effort.

G-protein–coupled receptors (GPCRs) are one of the most important classes of proteins in living organisms that allows transmission of a wide variety of signals over the cell membrane, between cells and over long distances in the human body, thus acting as both the gatekeepers and molecular messengers of the cell. The importance of these receptors is emphasized by the Nobel prize awarded in 2004 for the discoveries of "odorant receptors and the organization of the olfactory system" and in 2012 for "studies of G-protein–coupled receptors".

We wanted to use the Snifferomyces to address a real life challenge affecting several lives. Lack of diagnostic capacity has been a crucial barrier preventing an effective response to the challenges of Tuberculosis in low- and middle-income countries where, standard TB diagnostic tools that need to be used in a lab setting pose major barriers for screening due to the costs and time involved in the process. We aim to address this problem by constructing a diagnosis system for the presence of tuberculosis bacteria in the lungs via sensing of a chemical compound methyl nicotinate.





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