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Team:TMU-Tokyo/Project device3
From 2012.igem.org
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Device3 is composed of the strongest constitutive promoter (<A Href="http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100">BBa_J23100</A>), the srtongest RBS (<A Href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</A>) and <i>fdh4AB</i> formate dehydrogenase gene from <i>Methylobacterium extorquens</i>.<Br> | Device3 is composed of the strongest constitutive promoter (<A Href="http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100">BBa_J23100</A>), the srtongest RBS (<A Href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</A>) and <i>fdh4AB</i> formate dehydrogenase gene from <i>Methylobacterium extorquens</i>.<Br> | ||
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Formate dehydrogenase in <i>Methylobacterium extorquens</i> decomposes formate to water and corbon dioxide. This enzyme is a part of the methanol metabolism pathway.<Br> | Formate dehydrogenase in <i>Methylobacterium extorquens</i> decomposes formate to water and corbon dioxide. This enzyme is a part of the methanol metabolism pathway.<Br> | ||
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<i>FDH4AB</i> is composed of 2 subunits, the large subunit <i>FDH4A</i> and the small subunit <i>FDH4B</i>. In <i>Methylobacterium extorquens</i>, some similar enzymes, <i>FDH1, FDH2, FDH3</i> exist.<Br> | <i>FDH4AB</i> is composed of 2 subunits, the large subunit <i>FDH4A</i> and the small subunit <i>FDH4B</i>. In <i>Methylobacterium extorquens</i>, some similar enzymes, <i>FDH1, FDH2, FDH3</i> exist.<Br> | ||
The reason we selected <i>FDH4AB</i> from <i>FDH</i>s is that <i>FDH4AB</i> was the core enzyme in <i>FDH</i>s. In other wards, FDH4AB works even without other FDHs, but other FDHs don't work without FDH4AB.<Br> | The reason we selected <i>FDH4AB</i> from <i>FDH</i>s is that <i>FDH4AB</i> was the core enzyme in <i>FDH</i>s. In other wards, FDH4AB works even without other FDHs, but other FDHs don't work without FDH4AB.<Br> | ||
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| + | <i>Escherichia coli</i> has similar function gene of this gene, named <i>hycBCDEFG</i> and <i>fdhF</i>, the former genes cord hydrogenase Ⅲ and the latter gene cords FDH-F subunit that binds to former. The complex is called formate dehydrogenase-H (FHD-H).<Br> | ||
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| + | First, we planned to use these genes, but they have too long length to insert high copy plasmid and produces hydrogen instead of water. Therefore, we selected <i>fdh4AB</i> in <i>Methylobacterium extorquens</i>.<Br> | ||
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Revision as of 18:14, 16 September 2012
Device3;
Producing formate dehydrogenase, changing formate to water and carbon dioxide
Device3 is composed of the strongest constitutive promoter (BBa_J23100), the srtongest RBS (BBa_B0034) and fdh4AB formate dehydrogenase gene from Methylobacterium extorquens.
Formate dehydrogenase in Methylobacterium extorquens decomposes formate to water and corbon dioxide. This enzyme is a part of the methanol metabolism pathway.
FDH4AB is composed of 2 subunits, the large subunit FDH4A and the small subunit FDH4B. In Methylobacterium extorquens, some similar enzymes, FDH1, FDH2, FDH3 exist.
The reason we selected FDH4AB from FDHs is that FDH4AB was the core enzyme in FDHs. In other wards, FDH4AB works even without other FDHs, but other FDHs don't work without FDH4AB.
Escherichia coli has similar function gene of this gene, named hycBCDEFG and fdhF, the former genes cord hydrogenase Ⅲ and the latter gene cords FDH-F subunit that binds to former. The complex is called formate dehydrogenase-H (FHD-H).
First, we planned to use these genes, but they have too long length to insert high copy plasmid and produces hydrogen instead of water. Therefore, we selected fdh4AB in Methylobacterium extorquens.
■About fdh4AB in Methylobacterium extorquens
Ludmila Chistoserdova, Gregory J. Crowther, Julia A. Vorholt, Elizabeth Skovran, Jean-Charles Portais, and Mary E. Lidstrom. 2007. "Identification of a Fourth Formate Dehydrogenase in Methylobacterium extorquens AM1 and Confirmation of the Essential Role of Formate Oxidation in Methylotrophy."
[PubMed]
■Formate dehydrogenase in E. coli
"Enzyme: formate dehydrogenase H" [EcoCyc]
