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Team:TMU-Tokyo/Notebook/Assay 3 Protocol and Result
From 2012.igem.org
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<p class="description"> | <p class="description"> | ||
<b>■Assay3 Result</b><Br> | <b>■Assay3 Result</b><Br> | ||
| + | 全て enzym なし 243418.6 84.37 | ||
| + | 全て 30 ５0μl 229371.2 80.88 | ||
| + | すべて 10 20μl 248294.2 83.9823 | ||
| + | WT 10 20μl 232791.6 83.691 | ||
</p> | </p> | ||
Revision as of 20:19, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Assay 3
■Assay3 Protocol
1The cells were cultured at 30 ℃ for 18 hours in medium
2Centrifuged 10000 × g 5min
3Remove the supernatant
4Wash the fungus
E. coli disrupted by sonicator in buffer of 50mM Tris-HCl pH7.2 .
Centrifuged 10000 × g at 4 ℃ so that the enzyme is not deactivated, the supernatant was harvested as crude enzyme solution (If you want to save is stored at 4 ℃ in a cold room)
■Assay3 Result
全て enzym なし 243418.6 84.37
全て 30 ５0μl 229371.2 80.88
すべて 10 20μl 248294.2 83.9823
WT 10 20μl 232791.6 83.691
