Team:Goettingen/Project/Lab Work Flow

From 2012.igem.org

(Difference between revisions)
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* Every equipment should be cleaned after using.
* Every equipment should be cleaned after using.
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=== Materials ===
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=== Purging ===
* All bottles and erlenmeyer flasks should be rinsed with water after usage. Then they are collected in the box.
* All bottles and erlenmeyer flasks should be rinsed with water after usage. Then they are collected in the box.
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* Spülliste aktualisieren und gegebenenfalls gereinigtes Material abholen und aus der Liste streichen. Damit sich im Görlich Lab nichts sammelt, soll diese Liste von jedem Mitglied bei Betreten des Demo Labs überprüft werden.
+
* Keep purging list up to date and if necessary pick up purified material and remove it from the list. In order to prevent accumulation of material in the Görlich lab every team member shall check this list when entering the Demo Lab.
=== Autoclaving ===
=== Autoclaving ===
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* S1-Festmüll in den Metallboxen soll wöchentlich 1x zum Autoklavieren ins Görlich Lab gebracht werden. Die geschlossenen Tüten entweder in die blauen Mülleimer oder in den rechten Autoklaven in der Spülküche legen. Neue S1 Plastiktüten befinden sich neben dem Autoklaven rechts.
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* S1-solid waste within the metal boxes shall be given to be autoclaved once a week in the Görlich Lab. Place closed waste bags either in the blue waste bins or in the right autoclave in the purging room. New S1 plastic bags can be found on the right of the autoclave.
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* S1-Flüssigmüll wird in einem großen Erlenmeyerkolben unter dem Abzug gesammelt; zusätzlich steht eine kleine Schottflasche zur Verfügung. Entsorgung muss noch mit den TAs aus dem Görlich Lab geklärt werden.
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* S1-liquid waste is collected in an Erlenmeyer flask under the hood; additionally a small glas bottle is taken when working without sterile benches.  
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* Autoklavierliste aktualisieren und gegebenenfalls gereinigtes Material abholen und aus der Liste streichen. Damit sich im Görlich / Eichele Lab nichts sammelt, soll diese Liste von jedem Mitglied bei Betreten des Demo Labs überprüft werden.
+
* Keep autoclave list up to date and if necessary pick up purified material and remove it from the list. In order to prevent accumulation of material in the Görlich lab every team member shall check this list when entering the Demo Lab.
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* Flüssigmedien: Eichele Lab (T5, 2nd); Dauer: 2 h; Max.: 2x 1000ml Schott bottles, 2x 500ml and 2x 100 ml; selbst autoklavieren
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* Liquid media: Eichele Lab (T5, 2nd); Duration: 2 h; Max.: 2x 1000 mL Schott bottles, 2x 500 mL and 2x 100 mL; autoclave by yourself
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* Flüssig-/Feststoffe: Görlich Lab (T3, 2nd); TA autoklaviert für uns. Täglich ca. 9 Uhr
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* Liquid / Solid material: Görlich Lab (T3, 2nd); technician autoclaves. Daily ca. 9 am
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* Schottflaschen nicht ganz voll machen, erst recht NICHT, wenn Agar mit drin ist: 1000 mL -> 800-900 mL; 500 mL -> 400 mL
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* Do not fill Schott bottles completely, all the more NOT, if agar is added: 1000 mL -> 800-900 mL; 500 mL -> 400 mL
=== List of materials ===
=== List of materials ===
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* Einkäufe aus dem Chemikalienlager sollen im Wiki eingetragen werden: chronologisch und alphabetisch (gegebenfalls das Materialbuch fotografieren.)
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* Purchases from the chemical store shall be entered in the internal wiki: chronologically and alphabetically. (If necessary photograph the material book!)
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* Ins Materialbuch Datum und Kürzel zusätzlich eintragen, damit Einkäufe uns besser zugeordnet werden können.
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* Enter date and initials into the material book to support an easier assignment of purchases.
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* Einkäufe mit Datum, Preis und iGEM kennzeichnen und am passenden Ort lagern.
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* Mark purchases with date, price and iGEM and store them at appropriate place.
=== Working with EtBr ===
=== Working with EtBr ===
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== Daily Checklist ==
== Daily Checklist ==
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=== If you come to the lab ... ===
+
=== If you enter the lab ... ===
* Check the pick list of autoclaved materials
* Check the pick list of autoclaved materials
=== Check if the following things are present in a sufficient manner ===
=== Check if the following things are present in a sufficient manner ===

Revision as of 18:14, 25 September 2012

Deutsch  / English 

Contents

Golden Lab Rules

Rules for an organized Labflow!

Sample labeling

  • On the base of label it should be possible to find the sample in the corresponding notebook. For standardization all samples should include the following information:
  1. Date
  2. Description of content (DNA-, RNA-, protein)
  3. Concentration (if measured)
  4. Method (Gelex.; Clean-up; Ligation (Lig.), Restriction (Restr.), etc.)
  5. Volume
  • Every group was assigned to a color:
    • Group #1 blue
    • Group #2 green
    • Group #3 red

Notebook

  • Language: English
  • Note everything you have done the day and what you have prepared for the following day (e.g. inoculation of cultures).
  • Note storage location.
  • Important methods can be marked with "Post-its".

Workplace

  • Every day all used workplaces should be cleaned, especially the clean-bench should be cleaned with 70% EtOH.
  • Do not use the clean-bench for storage. For enough space and a working air circulation keep the clean-bench free of bulky equipment.
  • Every equipment should be cleaned after using.

Purging

  • All bottles and erlenmeyer flasks should be rinsed with water after usage. Then they are collected in the box.
  • Keep purging list up to date and if necessary pick up purified material and remove it from the list. In order to prevent accumulation of material in the Görlich lab every team member shall check this list when entering the Demo Lab.

Autoclaving

  • S1-solid waste within the metal boxes shall be given to be autoclaved once a week in the Görlich Lab. Place closed waste bags either in the blue waste bins or in the right autoclave in the purging room. New S1 plastic bags can be found on the right of the autoclave.
  • S1-liquid waste is collected in an Erlenmeyer flask under the hood; additionally a small glas bottle is taken when working without sterile benches.
  • Keep autoclave list up to date and if necessary pick up purified material and remove it from the list. In order to prevent accumulation of material in the Görlich lab every team member shall check this list when entering the Demo Lab.
  • Liquid media: Eichele Lab (T5, 2nd); Duration: 2 h; Max.: 2x 1000 mL Schott bottles, 2x 500 mL and 2x 100 mL; autoclave by yourself
  • Liquid / Solid material: Görlich Lab (T3, 2nd); technician autoclaves. Daily ca. 9 am
  • Do not fill Schott bottles completely, all the more NOT, if agar is added: 1000 mL -> 800-900 mL; 500 mL -> 400 mL

List of materials

  • Purchases from the chemical store shall be entered in the internal wiki: chronologically and alphabetically. (If necessary photograph the material book!)
  • Enter date and initials into the material book to support an easier assignment of purchases.
  • Mark purchases with date, price and iGEM and store them at appropriate place.

Working with EtBr

  • Work only on places which are denoted for working with EtBr. Following equipments are included: UV-table, EtBr-basin, bleach-basin, transport-basin, scalpels, Pasteur-pipette and EtBr-bottle
  • Do not contaminate gel-chambers or power supply.


Daily Checklist

If you enter the lab ...

  • Check the pick list of autoclaved materials

Check if the following things are present in a sufficient manner

  • Cupboard of autoclaving materials
    • E-Cups, PCR-Tubes
    • Media: LB (with agar & liquid)
    • Test tubes
    • Pipette-tips
    • LB-agar plates
  • Other consumables
    • Petri dishes (small/big)
    • Tips
    • Gloves
    • Chemicals
    • Restock 70% EtOH-bottles

In between...

  • Tidy up the fridge
  • Dispose of waste (at the latest on thursday)
  • Gel-Bench: enough TAE (1x & 50x), agarose?

If you leave the lab...

  • Turn of all equipments.
  • Clean-bench closed??
  • Switch off the light.



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