Team:BostonU/Project Overview

• Our Team
  • Team
  • Summer Fun
• Project
  • Project Overview and Abstract
  • Introduction to Characterization
  • Introduction to MoClo
  • Methodology Overview
  • Results Summary
• Achievements
  • Data Collected
  • Parts Submitted
  • MoClo Kit
  • Medal Fulfillment
• Notebook
  • Protocols
  • Clotho and Eugene
  • Weekly Notebook
• Considerations
  • Synthetic Biology Advancement
  • New England iGEM Regional Meeting
  • Human Practices
  • Safety
• Acknowledgements
  • Collaborations
  • Acknowledgements

Project Overview

Overall goals: Our major goal is to generate a standard protocol for the characterization of genetic circuits containing fluorescent proteins and share this protocol with the synthetic biology community. Our secondary goal is to introduce MoClo as an alternative assembly method for iGEM teams to use.

In order to achieve this goal, there are many steps that must be taken:

• Convert BioBrick Parts into MoClo Parts
• Build Genetic Circuits with MoClo Parts
• Characterize Circuits using Flow Cytometry
• Generate Data Sheet for MoClo Parts

Abstract

Abandon All Hope, Ye Who PCR: MoClo and the Quest for Genetic Circuit Characterization

Monique De Freitas¹, Shawn Jin¹, Evan Appleton², Swati Carr², Sonya Iverson², Traci Haddock³, and Douglas Densmore^§

¹iGEM Team Member, ²iGEM Team Mentor, ³iGEM Team Advisor, ^§Faculty Sponsor, Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA