Team:Bielefeld-Germany/Results/trametis

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(TVEL0 Activity Tests)
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ZUsammenfassung
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[http://www.sigmaaldrich.com/catalog/product/sigma/51639?lang=de&region=DE TVEL0] was characterized in terms of its activity to establish activity test protocols and to create a standard which can be used as a reference.
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==TVEL0 Activity Tests==
==TVEL0 Activity Tests==
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[http://www.sigmaaldrich.com/catalog/product/sigma/51639?lang=de&region=DE TVEL0] was characterized in terms of its activity to establish activity test protocols and to create a standard which can be used as a reference.
 
===Initial Activity Test===
===Initial Activity Test===
[[File:Bielefeld2012_TVEL0_initial.jpg|thumbnail|450px|left|'''Figure 1:''']]
[[File:Bielefeld2012_TVEL0_initial.jpg|thumbnail|450px|left|'''Figure 1:''']]
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To standardize activity test methods used for this project a [http://www.sigmaaldrich.com/catalog/product/sigma/51639?lang=de&region=DE laccase from ''Trametes versicolor''] (TVEL0) was used. The optimal composition for activity measurements contains 140 µL of 0.03 mg mL<sup>-1</sup> concentrated TVEL0 laccase solution, 100 mM sodium acetate buffer, 0.1 mM ABTS, ad 200 µL H<sub>2</sub>O. With this approach activity in oxidizing ABTS of TVEL0 was measured over a time period of 5 minutes at 25°C. The saturation of the reaction was reached after 3 minutes when ~80% ABTS got oxidized (see figure 1). This result proofed the activity measurement method and can therefor be used as a positive control.
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===Optimal pH of TVEL0===
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Revision as of 18:56, 26 September 2012

Laccase from Trametes versicolor

Summary

[http://www.sigmaaldrich.com/catalog/product/sigma/51639?lang=de&region=DE TVEL0] was characterized in terms of its activity to establish activity test protocols and to create a standard which can be used as a reference.


Contents


TVEL0 Activity Tests

Initial Activity Test

Figure 1:

To standardize activity test methods used for this project a [http://www.sigmaaldrich.com/catalog/product/sigma/51639?lang=de&region=DE laccase from Trametes versicolor] (TVEL0) was used. The optimal composition for activity measurements contains 140 µL of 0.03 mg mL-1 concentrated TVEL0 laccase solution, 100 mM sodium acetate buffer, 0.1 mM ABTS, ad 200 µL H2O. With this approach activity in oxidizing ABTS of TVEL0 was measured over a time period of 5 minutes at 25°C. The saturation of the reaction was reached after 3 minutes when ~80% ABTS got oxidized (see figure 1). This result proofed the activity measurement method and can therefor be used as a positive control.

Optimal pH of TVEL0

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