Team:Bielefeld-Germany/Results/Summary
From 2012.igem.org
Summary
BPUL - Laccase from Bacillus pumilus DSM 27 (ATCC7061)
After the measurement of activity of BPUL we made a scale-up and fermented E. coli KRX with <partinfo>BBa_K863000</partinfo> in Braun Biostat B with a total volume 3 L. Agitation speed, pO2 and OD600 were determined and illustrated in the following graph. We got a long lag phase of 2 hours due to a relativly old preculture. Then cell growth caused a decrease in pO2 and after 3 hours the value was below 50 %, so that the agitation speed was increased automatically. After 8,5 hours the deceleration phase started and therefore the agitation speed was descreased. The maximal OD600 of 3,53 was reached after 10 hours. The cells were harvested after 12 hours.
ECOL - Laccase from Escherichia coli BL21 (DE3)
XCCL - Laccase from Xanthomonas campestris pv. campestris B100
BHAL - Laccase from Bacillus halodurans C-125
TTHL - Laccase from Thermus thermophilus HB27
PCIL - Laccase from Pycnoporus cinnabarinus
TVEL5 - Laccase from Trametes versicolor
TVEL10 - Laccase from Trametes versicolor
TVEL13 - Laccase from Trametes versicolor
TVEL20 - Laccase from Trametes versicolor
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