Team:Bielefeld-Germany/Results/Summary

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(TTHL - Laccase from Thermus thermophilus HB27)
(BPUL - Laccase from Bacillus pumilus DSM 27 (ATCC7061))
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== BPUL - Laccase from ''Bacillus pumilus  DSM 27 (ATCC7061)'' ==
== BPUL - Laccase from ''Bacillus pumilus  DSM 27 (ATCC7061)'' ==
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[[File:Bielefeld2012_BPUL3LFermentation.jpg|600px|thumb|left|Fermentation of ''E.coli'' KRX with BBa_K863000 (BPUL) in 3L Fermenter]]
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[[File:Bielefeld2012_BPUL3LFermentation.jpg|600px|thumb|left|Fermentation of ''E.coli'' KRX with BBa_K863000 (BPUL) in Braun Biostat B, scale: 3&nbsp;L, autoinduction medium + 60&nbsp;µg/mL chloramphenicol, 37&nbsp;°C, pH&nbsp;7, agitation on cascade to hold a pO<sub>2</sub> of 50&nbsp;%, OD<sub>600</sub> taken every 30&nbsp;minutes.]]
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[[File:Bielefeld2012_BPUL6LFermentation.jpg|600px|thumb|left|Fermentation of ''E.coli'' KRX with BBa_K863000 (BPUL) in 6L Fermenter]]
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[[File:Bielefeld2012_BPUL6LFermentation.jpg|600px|thumb|left|Fermentation of ''E.coli'' KRX with BBa_K863000 (BPUL) in Bioengineering NFL22, scale: 6&nbsp;L, autoinduction medium + 60&nbsp;µg/mL chloramphenicol, 37&nbsp;°C, pH&nbsp;7, agitation increased when pO<sub>2</sub> was below 30&nbsp;%, OD<sub>600</sub> taken every hour. ]]
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Revision as of 10:19, 23 September 2012

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Contents

Summary

BPUL - Laccase from Bacillus pumilus DSM 27 (ATCC7061)

Fermentation of E.coli KRX with BBa_K863000 (BPUL) in Braun Biostat B, scale: 3 L, autoinduction medium + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold a pO2 of 50 %, OD600 taken every 30 minutes.


Fermentation of E.coli KRX with BBa_K863000 (BPUL) in Bioengineering NFL22, scale: 6 L, autoinduction medium + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation increased when pO2 was below 30 %, OD600 taken every hour.


ECOL - Laccase from Escherichia coli BL21 (DE3)

Fermentation of E.coli KRX with BBa_K863005 (ECOL) in 3L Fermenter


Fermentation of E.coli KRX with BBa_K863005 (ECOL) in 6L Fermenter


XCCL - Laccase from Xanthomonas campestris pv. campestris B100

BHAL - Laccase from Bacillus halodurans C-125

TTHL - Laccase from Thermus thermophilus HB27

Fermentation of E.coli KRX with BBa_K863012 (TTHL) in 6L Fermenter


PCIL - Laccase from Pycnoporus cinnabarinus

TVEL5 - Laccase from Trametes versicolor

TVEL10 - Laccase from Trametes versicolor

TVEL13 - Laccase from Trametes versicolor

TVEL20 - Laccase from Trametes versicolor

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