Team:Bielefeld-Germany/Project/Background/coli

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 [https://2012.igem.org/Team:Bielefeld-Germany/Project/Background zurück]
-==''Escherichia coli''==
-Another laccase, which catched our attention is the enzyme [http://www.ncbi.nlm.nih.gov/protein/85674340 CueO] from ''Escherichia coli''. The bacterium naturally occurs in human and animal intestine. In laboratory it’s a model organism and the most widely used organism in molecular genetics. So the about 1-6 µm long all rounder is even more promising and has a laccase with a high potential for our approaches. It is described that CueO is able to oxidize different PAHs (Zeng, Lin et al., 2011). Our aim is to produce and characterize this laccase with regard to the plan to develop a biological filter using immobilized laccases. Therefore we isolated the laccase CueO from ''E. coli DE''(BL21) out of genomic DNA. For further information about the characterization of CueO [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary look here].
-Zeng, J., X. Lin, et al. (2011). "Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from ''E. coli''." Appl Microbiol Biotechnol 89(6): 1841-1849.
-{{Team:Bielefeld/Sponsoren}}