Team:Bielefeld-Germany/Project/Achievments

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Project Achievements

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We successfully produced purified and identified active laccases of E. coli BL21(CueO named ECOL), Bacillus pumilus DSM 27 laccase (CotA named BPUL), Bacillus halodurans C-125 laccase (Lbh1 named BHAL) and Thermus thermophilus HB27 (tthL named TTHL).

Results ECOL Results BPUL Results TTHL Results BHAL

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We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest.

Results


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Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.

Results ECOL Results BPUL Results TTHL Results BHAL


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We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.

Results


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We successfully immobilized Trametes versicolor-laccase (named TVEL0), E. coli BL21-laccase (CueO named ECOL) and Bacillus pumilus DSM 27-laccase (CotA named BPUL) and measured their activity.

Results TVEL0 Results ECOL Results BPUL


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We successfully improved a BioBrick by clarifying its true function.

[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]


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We successfully created our own database to organize our samples.

Results


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We successfully created a model which simulates the oxidation of ABTS.

Results


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We conducted a feasibility study of our project with sewage plant experts.

Results


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We did an outstanding outreach work.

Results



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