Team:Bielefeld-Germany/Project/Achievments

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Project Achievements

We successfully produced purified and identified active laccases of E. coli BL21(CueO named ECOL), Bacillus pumilus DSM 27 laccase (CotA named BPUL), Bacillus halodurans C-125 laccase (Lbh1 named BHAL) and Thermus thermophilus HB27 (tthL named TTHL).
ECOL/coli Results BPUL/pumi Results TTHL/thermo Results BHAL/halo Results

We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest.
Results

Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
ECOL/coli#Activity_Analysis_of_ECOL Results BPUL/pumi#Activity_analysis_of_BPUL Results TTHL/thermo#Activity_analysis_of_TTHL Results BHAL/halo#Activity_Analysis_of_BHAL Results

We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.
Results

We successfully immobilized Trametes versicolor-laccase (named TVEL0), E. coli BL21-laccase (CueO named ECOL) and Bacillus pumilus DSM 27-laccase (CotA named BPUL) and measured their activity.
TVEL0/immo Results ECOL/immo Results BPUL/immo Results

We successfully improved a BioBrick by clarifying its true function.
Results

We successfully created our own database to organize our samples.
Results

We successfully created a model which simulates the oxidation of ABTS.
Results

We conducted a feasibility study of our project with sewage plant experts.
Results

We did an outstanding outreach work.
Results

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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info(at)igem-bielefeld.de

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