Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced a active ''Bacillus halodurans'' C-125e -Laccase (Lbh1 named BHAL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced a active ''Bacillus halodurans'' C-125e -Laccase (Lbh1 named BHAL) [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a methode for degradation with our self produced laccases. The LC-MS and HPLC analytics showed that our laccaes are able to degradate the hormonal substrates; Estradiol, Esteron and Ethinyl estradiol [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a methode for degradation with our self produced laccases. The LC-MS and HPLC analytics showed that our laccaes are able to degradate the hormonal substrates; Estradiol, Esteron and Ethinyl estradiol [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of Bacillus pumilus DSM 27 -Laccase (CotA named BPUL)  [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of E. coli BL21- Laccase (CueO named ECOL)  [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] Successful immobilization of Trametes versicolor  - Laccase (named TVEL0)
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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Revision as of 22:26, 26 September 2012


Project Achievments

Bielefeld2012 haken grau.jpg
We successfully produced purified and identified a active E. coli BL21- Laccase (CueO named ECOL) Results


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest.Results


Bielefeld2012 haken grau.jpg
We successfully produced purified and identified a active Bacillus pumilus DSM 27 -Laccase (CotA named BPUL) Results


Bielefeld2012 haken grau.jpg
We successfully produced, purified a active Thermus thermophilus HB27- Laccase (tthL named TTHL) Results


Bielefeld2012 haken grau.jpg
We successfully produced a active Bacillus halodurans C-125e -Laccase (Lbh1 named BHAL) Results


Bielefeld2012 haken grau.jpg
We estabilished a methode for degradation with our self produced laccases. The LC-MS and HPLC analytics showed that our laccaes are able to degradate the hormonal substrates; Estradiol, Esteron and Ethinyl estradiol Results


Bielefeld2012 haken grau.jpg
Successful immobilization of Bacillus pumilus DSM 27 -Laccase (CotA named BPUL) Results


Bielefeld2012 haken grau.jpg
Successful immobilization of E. coli BL21- Laccase (CueO named ECOL) Results


Bielefeld2012 haken grau.jpg
Successful immobilization of Trametes versicolor - Laccase (named TVEL0)

Results




55px Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg