Team:Bielefeld-Germany/Modell

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(Model of a fixed-bed reactor)
(Model of a fixed-bed reactor)
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==Model of a fixed-bed reactor==
==Model of a fixed-bed reactor==
In our project we plan to construct a fixed-bed reactor, where immobilized laccases degrade synthetic estrogen and other harmful substances. As a small selection we plan to caracterize our different laccases for  three estrogens, three analgesics, four PAH´s, one insecticide and three possible redox mediators.
In our project we plan to construct a fixed-bed reactor, where immobilized laccases degrade synthetic estrogen and other harmful substances. As a small selection we plan to caracterize our different laccases for  three estrogens, three analgesics, four PAH´s, one insecticide and three possible redox mediators.
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If we could model the degradation of one substrate by one Laccase, we could easily replace the specific Kcat/Km quotient for another Laccase andand the amounts oft he other substrates.
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If we could model the degradation of one substrate by one Laccase, we could easily replace the specific K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> quotient for another Laccase and the amounts of the other substrates.
   
   
We ignore the possible cofactors ABTS, syringaldazine and viuloric acid, because on the one hand thy would cause increase the costs, and one the other hand they are harmful substances too. As shown by Team Substrate Analytic TVEL0 degrades ethinyl estradiol without reox mediator. So the reaction should follow the michaelis menten kinetics.
We ignore the possible cofactors ABTS, syringaldazine and viuloric acid, because on the one hand thy would cause increase the costs, and one the other hand they are harmful substances too. As shown by Team Substrate Analytic TVEL0 degrades ethinyl estradiol without reox mediator. So the reaction should follow the michaelis menten kinetics.

Revision as of 17:56, 26 September 2012

Modelling

Contents

Model of a fixed-bed reactor

In our project we plan to construct a fixed-bed reactor, where immobilized laccases degrade synthetic estrogen and other harmful substances. As a small selection we plan to caracterize our different laccases for three estrogens, three analgesics, four PAH´s, one insecticide and three possible redox mediators. If we could model the degradation of one substrate by one Laccase, we could easily replace the specific Kcat KM-1 quotient for another Laccase and the amounts of the other substrates.

We ignore the possible cofactors ABTS, syringaldazine and viuloric acid, because on the one hand thy would cause increase the costs, and one the other hand they are harmful substances too. As shown by Team Substrate Analytic TVEL0 degrades ethinyl estradiol without reox mediator. So the reaction should follow the michaelis menten kinetics.

The reaction

File:Bielefeld2012 MM.png This transformation can be found in "Stryer biochemie". This formula is suitable for very low substrate concentrations. In this case we can estimate the substrate concentrations to 1.89 ng L-1.

Our Model with different Kcat KM-1 values. The span differs from 0.1 seconds to 1500 seconds.

Extend the model with sewageplant data

Sewageplant data from Schlossholte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s-1, maximum 232.8 L s-1 and avarage 76.8 L s-1


The required enzyme amount for a sewageplant is dependent on Kcat KM-1 and the flow rate



Outlook

"Team Substrate Analytic" will determine Kcat KM-1. Additionaly we have the opportunity to work with a lab sewageplant. So we´re going to test our model in defined conditions.

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