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Team:Bielefeld-Germany/Labjournal/week6
From 2012.igem.org
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14] | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14] | ||
[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15] | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15] | ||
| + | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16 Week16] | ||
| + | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17 Week17] | ||
| + | [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18 Week18] | ||
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==Week 6 (06/04 - 06/10/12)== | ==Week 6 (06/04 - 06/10/12)== | ||
Revision as of 19:20, 16 August 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 6 (06/04 - 06/10/12)
Monday June 4th
- Primerdesign for isolating a laccase from Arababidopsis thaliana cDNA. Since we want to express the laccase in E.coli we designed the primers like before for the bacterial laccases with T7 promotor and HIS-tag.
Tuesday June 5th
- Team Activity Tests: After testing the T. versicolor laccase under conditions that are optimal (pH 5, 25°C ) according to the literature we now started further characterization under different pHs. We analyzed the laccases behavior when working in 100 mM natrium acetate buffer at pH 1, 3, 7 an 9. Result: We agree with the literature that pH 5 and also pH 6 seem to make the laccase happy. Since not all waste waters (especially those here in Germany) are not as warm as 25°C we now wonder what our laccase might do when exposed to low temperature such as 4°C. Stay tuned.
Wednesday June 6th
Thursday June 7th
- Team Modeling: becomeing acquainted with matlab while reading the manual
