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Team:Bielefeld-Germany/Labjournal/week4
From 2012.igem.org
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| - | *''' Team Student Academy:''' Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. | + | *''' Team Student Academy:''' Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. For setup see [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th here]. Got in both cases intense red fluorescing colonies but no green fluorescing colonies. Asking for other plasmids containing GFP at the working groups of our University. |
===Thursday May 24th=== | ===Thursday May 24th=== | ||
Revision as of 14:59, 24 August 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 4 (05/21 - 05/27/12)
Monday May 21st
- Team Modeling: Our aims for modeling:
- model the expression of the laccases in the organisms.
- model the aktivity of our enzyms.
- model the interesing parts of a clarification plant (the part witch are interesing for our cleaner.
Tuesday May 22nd
- Successful PCRs of laccase genes from Thermus thermophilus HB27 and Bacillus halodurans C-125 with genomic DNA as template. The primers were designed with T7-Promotor-overhanging ends after prefix and HIS-Tag-overhang before suffix.
Wednesday May 23rd
- Team Student Academy: Repetition of the transformation of BBa_J04450 and BBa_I13522 with new competent cells. For setup see here. Got in both cases intense red fluorescing colonies but no green fluorescing colonies. Asking for other plasmids containing GFP at the working groups of our University.
Thursday May 24th
- Team Student Academy: Made a liquid culture of fluorescing BBa_J04450 colonies at 30°C. There was no fluorescence.
