Team:Bielefeld-Germany/Labjournal/week3

From 2012.igem.org

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(Wednesday May 16th)
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===Thursday May 17th===
===Thursday May 17th===
* '''Team Modeling''': Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
* '''Team Modeling''': Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
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* '''Team Bacterial Laccases''': Picking colonies from the transformed BBa_J04450 plasmid and plating on new LB plates with chloramphenicol.
===Friday May 18th===
===Friday May 18th===
===Saturday May 19th===
===Saturday May 19th===
===Sunday May 20th===
===Sunday May 20th===

Revision as of 19:53, 12 September 2012

Contents

Labjournal

Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18 Week19 Week20 Week21


Week 3 (05/14 - 05/20/12)

weekly seminar:

  • first lab service: Robert
  • our GFP, which we wanted to use for the summer school for pupils, does not work
  • first competent cells have to be made: Julia S. and Robert
  • Decision to buy a commercial laccase to establish the analytics and the enzyme tests
  • our expose has to be translated into english: Malak
  • last planning for our waver sell
  • Julia S. is creating a vector for Pichia pastoris and is now looking for sequences
  • The [http://www.bmbf.de/en/index.php BMBF] invites all german iGEM teams to Berlin to attend at the Biotechnologie2020+ strategy process


Monday May 14th

Tuesday May 15th

  • Team Activity Tests: For some pre test and characterization for our future laccase activity standard we ordered [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de&region=DE laccase] from Trametes versicolor. As well we had to order a substrate that the laccase could use to demonstrate its abilities. According to the literature [http://www.sigmaaldrich.com/catalog/product/sigma/a1888?lang=de&region=DE ABTS] is a well working substrate to characterize oxidizing enzym activity. So we ordered.

Wednesday May 16th

  • Team Bacterial Laccases: For cloning our laccases we need pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in competent KRX cells.

Thursday May 17th

  • Team Modeling: Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
  • Team Bacterial Laccases: Picking colonies from the transformed BBa_J04450 plasmid and plating on new LB plates with chloramphenicol.

Friday May 18th

Saturday May 19th

Sunday May 20th