Team:Bielefeld-Germany/Labjournal/week20
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** Cell disruption via high-pressure homogeniser and purification via Ni-NTA column were performed for the following samples: 3L fermentation of ''E.coli'' KRX with laccase from ''B.pumilus'' (09/09), 6L fermentation of ''E.coli'' KRX with laccase from ''E.coli'' (09/07) and ''B.pumilus'' (09/09). | ** Cell disruption via high-pressure homogeniser and purification via Ni-NTA column were performed for the following samples: 3L fermentation of ''E.coli'' KRX with laccase from ''B.pumilus'' (09/09), 6L fermentation of ''E.coli'' KRX with laccase from ''E.coli'' (09/07) and ''B.pumilus'' (09/09). | ||
** We made SDS-Pages of purification of laccase from ''B.pumilus'' | ** We made SDS-Pages of purification of laccase from ''B.pumilus'' | ||
- | + | * '''Team Site Directed Mutagenesis:''' | |
+ | **Digestion of the six tvel10-plasmids. Three were unmutated and three had pieces of DNA of the wrong size. | ||
===Tuesday September 11th=== | ===Tuesday September 11th=== |
Revision as of 20:40, 19 September 2012
Contents |
Week 20 (09/10 - 09/16/12)
Monday September 10th
- Team Cultivation & Purification:
- Cell disruption via high-pressure homogeniser and purification via Ni-NTA column were performed for the following samples: 3L fermentation of E.coli KRX with laccase from B.pumilus (09/09), 6L fermentation of E.coli KRX with laccase from E.coli (09/07) and B.pumilus (09/09).
- We made SDS-Pages of purification of laccase from B.pumilus
- Team Site Directed Mutagenesis:
- Digestion of the six tvel10-plasmids. Three were unmutated and three had pieces of DNA of the wrong size.
Tuesday September 11th
Wednesday September 12th
Thursday September 13th
Friday September 14th
Saturday September 15th
Sunday September 16th
Sunday
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