"
Team:Bielefeld-Germany/Labjournal/week17
From 2012.igem.org
(→Tuesday August 21st) |
(→Monday August 20th) |
||
| Line 26: | Line 26: | ||
===Monday August 20th=== | ===Monday August 20th=== | ||
* '''Team Site Directed Mutagenesis:''' | * '''Team Site Directed Mutagenesis:''' | ||
| - | + | Plasmidisolation of X.camp_2247 1-4 and X.camp_3633 1-4 | |
| - | + | Testrestriktion with PstI: - all negativ | |
| + | Colony-PCR of T10_NotI (24 Colonies) -all negativ | ||
| + | * '''Team Cellulose Binding Domain:''' | ||
| + | CBD: Colony-PCR of 24 Assembly-colonies - all colonies only have CBDclos. Assembly didn't work at all... | ||
===Tuesday August 21st=== | ===Tuesday August 21st=== | ||
Revision as of 17:42, 22 August 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 17 (08/20 - 08/26/12)
Monday August 20th
- Team Site Directed Mutagenesis:
Plasmidisolation of X.camp_2247 1-4 and X.camp_3633 1-4 Testrestriktion with PstI: - all negativ Colony-PCR of T10_NotI (24 Colonies) -all negativ
- Team Cellulose Binding Domain:
CBD: Colony-PCR of 24 Assembly-colonies - all colonies only have CBDclos. Assembly didn't work at all...
Tuesday August 21st
Wednesday August 22nd
Street Science: Went to Dr. Joe Max Risse today and asked him about the mircoorganisms of the Fermentationgroup. He recommended Euglena gracilis, since it is without risk, big, colorful, and very healthy under the microscop. I asked for the bacteria they have and he told me I could check if their Kocuria rosea is a wild type. In the DMSZ catalog there are three strains of Kocuria rosea and all have been assessed to be of riskgroup 1, which is the safest group of bacteria and means it is unlikely that it will infect humans. I also asked for Penicillium chrysogenum but the one they use is a GVO.
- Team Cellulose Binding Domain:
Test-restriktion of all isolated CBDcex- and the GFP_Freiburg-plasmids with NotI - no positiv result Started new and made a gradient PCR for GFP-Freiburg and CBDcex (50-70°C)
