Team:Bielefeld-Germany/Labjournal/week16
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Labjournal
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Week 16 (08/13 - 08/19/12)
Monday August 13th
Tuesday August 14th
- Team Arabidopsis Laccase: Today our cDNA took a bath in ethanol and got cleaned. We are pretty sure our next PCR will be a lot more successful. Check protocols for further information about the cDNA washing via ethanol precipitation.
Wednesday August 15th
- Team Wiki: We set up some more wiki rules. Today´s rules were about citations. We agreed von some standards that will for sure help make the wiki a little prettier. Have a look:
- Paper: Exampleman M et al. (2002). The example paper. The example journal (Volume): pages.
- Website: Name of website, URL, Index, date site visited
- Book: Examplewomen M et al. (1999). The example book. The example publisher (edition).
Next will be a standard for charts.
Thursday August 16th
Friday August 17th
Saturday August 18th
Sunday August 19th
- Team Site Directed Mutagenesis:
Plasmidisolation of X.camp_2247- 1-4 and _3633 1-4 Test-restriktion of X.camp_2247 1-4 and _3633 1-4 with PstI for 3 hours 2247:1,2+4 no Mutation 3633:2+3 no Mutation. 2247-3 and 3633-1 only cut once (should be twice)
Picked 4 new colonys per site and plated the on CM-agar
Colony-PCR of 10 Colonies of T10 1,2,3,4 bands at 1100 - should be RFP 9 + 10 bands could be higher but not 1600 as needed… plated anyway for Test-restriktion.