Team:Bielefeld-Germany/Labjournal/week16

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=Labjournal=
 
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1 Week1]
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<html>
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2 Week2]
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<style type="text/css">
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3 Week3]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4 Week4]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5 Week5]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6 Week6]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7 Week7]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8 Week8]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9 Week9]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10 Week10]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11 Week11]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12 Week12]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13 Week13]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16 Week16]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17 Week17]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18 Week18]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19 Week19]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20 Week20]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21 Week21]
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==Week 16 (08/13 - 08/19/12)==
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ul {list-style-image:none;}
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#bodyContent{
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}
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===Monday August 13th===
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</style>
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===Tuesday August 14th===
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* '''Team Arabidopsis Laccase''': Today our cDNA took a bath in ethanol and got cleaned. We are pretty sure our next PCR will be a lot more successful. Check protocols for further information about the cDNA washing via [https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Genetics#Ethanol_precipitation_to_clean_DNA ethanol precipitation].
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===Wednesday August 15th===
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<!-- navigator -->
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*'''Team Wiki''': We set up some more wiki rules. Today´s rules were about citations. We agreed von some standards that will for sure help make the wiki a little prettier. Have a look:
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<div id="nav" class="tabs">
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** <span style="text-decoration: underline;">Paper</span>: Exampleman M ''et al''. (2002). The example paper. The example journal (Volume): pages.
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<div class="scroller">
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** <span style="text-decoration: underline;">Website</span>: Name of website, URL, Index, date site visited
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<ul style="list-style-type:none">
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** <span style="text-decoration: underline;">Book</span>: Examplewomen M ''et al''. (1999). The example book. The example publisher (edition).
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/Starting"><strong>Prologue</strong></a></li>
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Next will be a standard for charts.
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1"><strong>Week 1</strong></a></li>
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2"><strong>Week 2</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3"><strong>Week 3</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4"><strong>Week 4</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5"><strong>Week 5</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6"><strong>Week 6</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7"><strong>Week 7</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8"><strong>Week 8</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9"><strong>Week 9</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10"><strong>Week 10</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11"><strong>Week 11</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12"><strong>Week 12</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13"><strong>Week 13</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14"><strong>Week 14</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15"><strong>Week 15</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16"><strong>Week 16</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17"><strong>Week 17</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18"><strong>Week 18</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19"><strong>Week 19</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20"><strong>Week 20</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"><strong>Week 21</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week22"><strong>Week 22</strong></a></li>
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===Thursday August 16th===
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</ul>
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===Friday August 17th===
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===Saturday August 18th===
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* '''Team bacterial laccases''': Today we performed a ligation from the digestions we have done before. The ligation included of course the T7 promotor, the backbone pSB1C3 and the individual laccase genes. Ligation was performed with T4 ligase for 20 minutes at room temperature and stopped through a five minute incubation at 70°C.
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===Sunday August 19th===
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</div>
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</div>
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</html>
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* '''Team Site Directed Mutagenesis:'''
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<div style="text-align:justify;">
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Plasmidisolation of X.camp_2247- 1-4 and _3633 1-4
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==Week 16 (08/13 - 08/19/12)==
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Test-restriktion of  X.camp_2247 1-4 and _3633 1-4 with PstI for 3 hours
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2247:1,2+4 no Mutation
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3633:2+3 no Mutation.
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2247-3 and 3633-1 only cut once (should be twice)
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Picked 4 new colonys per site and plated the on CM-agar
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Colony-PCR of 10 Colonies of T10
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{{Team:Bielefeld/Sponsoren}}
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1,2,3,4 bands at 1100 - should be RFP
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9 + 10 bands could be higher but not 1600 as needed… plated anyway for Test-restriktion.
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Latest revision as of 21:26, 25 September 2012


Week 16 (08/13 - 08/19/12)

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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